Customising Chassis Cells for Structured Meat Products
For cultivated meat R&D teams, producing structured whole-cuts like steaks or fillets requires more than just growing cells. The key lies in chassis cells - muscle, fat, and connective tissue...
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Defined Bioscience | SKU:
Defined Bioscience FGF2-G3 Growth Factor (Bovine)
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Defined Bioscience’s FGF2-G3 (Bovine) is a thermostabilised recombinant version of bovine fibroblast growth factor 2 engineered for use in cultivated-meat cell culture. It supports proliferation and maintenance of bovine muscle and stem cells with minimal medium supplementation. The enhanced thermostability of this variant ensures consistent activity for extended periods at 37 °C, eliminating the need for daily or weekend media changes and reducing process variability in long-term culture.
The protein sequence is derived from Bos taurus FGF2 (UniProt P03969) with nine key amino-acid substitutions introduced to improve thermostability and bioactivity. These modifications mirror those proven effective in the human FGF2-G3 variant, resulting in identical stability benefits. FGF2-G3 (Bovine) is recombinantly expressed in E. coli, animal-origin-free, and purified to greater than 95 % purity as confirmed by SDS-PAGE. It exhibits an EC₅₀ below 1 ng/mL in NIH-3T3 proliferation assays, indicating high potency at reduced concentrations compared with native FGF2.
Supplied as either a sterile 1 mg/mL liquid or lyophilised powder, the protein can be reconstituted into standard buffer formulations and substituted directly for wild-type FGF2 in serum-free and chemically defined media. Its species-specific sequence makes it ideal for bovine cell lines used in cultivated-beef development and scale-up research.
For research and development use only. Not for human or diagnostic applications.
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Scale compatibilityProduction scale
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Animal origin freeYes
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Buffer additives allowedYes
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Chemically definedYes
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Mix immediately after thawYes
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Xeno‑freeYes
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Cellag Bio is the structured intelligence layer for cultivated meat R&D — scattered published research distilled into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before committing budget to a medium, reagent, or cell line, check the evidence behind it: real experimental outcomes and failure modes, not marketing claims.
Search by media, reagent, cell line or species (e.g. "Beefy-9" or "B8") to find the records that use it.
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