Worlds First Cultivated Meat B2B Marketplace: Read Announcement
In stock. Ships globally direct from Defined Bioscience to research and production facilities.
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Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Defined Bioscience provides direct post-sales support including troubleshooting, process optimisation, and protocol guidance. Batch-specific documents available on request.
Defined Bioscience’s FGF2-G3 (Bovine) is a thermostabilised recombinant version of bovine fibroblast growth factor 2 engineered for use in cultivated-meat cell culture. It supports proliferation and maintenance of bovine muscle and stem cells with minimal medium supplementation. The enhanced thermostability of this variant ensures consistent activity for extended periods at 37 °C, eliminating the need for daily or weekend media changes and reducing process variability in long-term culture.
The protein sequence is derived from Bos taurus FGF2 (UniProt P03969) with nine key amino-acid substitutions introduced to improve thermostability and bioactivity. These modifications mirror those proven effective in the human FGF2-G3 variant, resulting in identical stability benefits. FGF2-G3 (Bovine) is recombinantly expressed in E. coli, animal-origin-free, and purified to greater than 95 % purity as confirmed by SDS-PAGE. It exhibits an EC₅₀ below 1 ng/mL in NIH-3T3 proliferation assays, indicating high potency at reduced concentrations compared with native FGF2.
Supplied as either a sterile 1 mg/mL liquid or lyophilised powder, the protein can be reconstituted into standard buffer formulations and substituted directly for wild-type FGF2 in serum-free and chemically defined media. Its species-specific sequence makes it ideal for bovine cell lines used in cultivated-beef development and scale-up research.
For research and development use only. Not for human or diagnostic applications.
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