Customising Chassis Cells for Structured Meat Products
For cultivated meat R&D teams, producing structured whole-cuts like steaks or fillets requires more than just growing cells. The key lies in chassis cells - muscle, fat, and connective tissue...
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Based on supplier data and compatible alternatives for this cell line. See Protocol Notes for details, then select the required components and add them alongside your order.
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A robust dermis-derived porcine fibroblast cell line isolated post-mortem (post-rigor) from a 6-month-old male pig sourced from an FSIS-inspected, USDA-regulated meat-processing facility in the United States. Donor animals were raised under an IACUC-approved protocol within an AAALAC-accredited program following Agriculture Guide standards, and tissues were collected and processed under institutional biosafety protocols into primary cells before cryopreservation.
The line was subsequently engineered via HDR-mediated targeted cassette integration, incorporating an immortalization factor and three pro-proliferative factors to enable long-term expansion and sustained growth performance. Cells are supplied frozen in a 1 mL cryovial (store in the vapor phase of liquid nitrogen; rapid thaw at 37°C recommended) and include a one-year research license.
A comprehensive documentation pack is provided, including donor and sourcing summary, characterization data, morphology images, long-term culture identity (karyotype and transcriptome PCA), recommended culture media, and storage/growth protocols, along with technical support for media optimization and process development; the license also includes ongoing media optimization updates and protocol refinements at no additional subscription cost.
For research use only, not for human or animal therapeutic use, diagnostic use, or consumption.
Cellag Bio is the structured intelligence layer for cultivated meat R&D — scattered published research distilled into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before committing budget to a medium, reagent, or cell line, check the evidence behind it: real experimental outcomes and failure modes, not marketing claims.
Search by media, reagent, cell line or species (e.g. "Beefy-9" or "B8") to find the records that use it.
Protocol Notes
About These Recommendations
Products shown in the Recommended Setup reflect how this cell line is typically cultured, based on supplier input and validated substitutions where no specific product is defined.
Compatibility is based on supplier data and reported use. Performance may vary depending on process conditions and scale.
These recommendations are intended as starting-point guidance and do not replace the supplier's handling protocol, which is provided after purchase.
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