Livestock Labs Porcine Fibroblasts

A robust dermis-derived porcine fibroblast cell line isolated post-mortem (post-rigor) from a 6-month-old male pig sourced from an FSIS-inspected, USDA-regulated meat-processing facility in the United States. Donor animals were raised under an IACUC-approved protocol within an AAALAC-accredited program following Agriculture Guide standards, and tissues were collected and processed under institutional biosafety protocols into primary cells before cryopreservation.

The line was subsequently engineered via HDR-mediated targeted cassette integration, incorporating an immortalization factor and three pro-proliferative factors to enable long-term expansion and sustained growth performance. Cells are supplied frozen in a 1 mL cryovial (store in the vapor phase of liquid nitrogen; rapid thaw at 37°C recommended) and include a one-year research license.

A comprehensive documentation pack is provided, including donor and sourcing summary, characterization data, morphology images, long-term culture identity (karyotype and transcriptome PCA), recommended culture media, and storage/growth protocols, along with technical support for media optimization and process development; the license also includes ongoing media optimization updates and protocol refinements at no additional subscription cost.

For research use only, not for human or animal therapeutic use, diagnostic use, or consumption.