Non-Animal Scaffolds Under Heat and pH Stress
For cultivated meat R&D teams, I assess scaffold stability by retained function - not appearance. Test your formulation through its actual heating, cooling and pH sequence. For adherent culture, check...
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Livestock Labs | SKU:
Livestock Labs Porcine Fibroblasts
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£3,737.00
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Supplier protocol
What the supplier's protocol for these cells calls for, and where to find it on Cellbase.
Basal medium
DMEM, high glucose, pyruvate
Protocol Specified
Gibco™ DMEM, high glucose, pyruvate
Direct Substitute
KCell DMEM
Choose the high-glucose formulation. Use with sodium pyruvate as an alternative to the specified medium. Not validated by Livestock Labs.
Serum
FBS
Protocol Specified
Gibco™ Fetal Bovine Serum
Supplements
Sodium pyruvate (with KCell DMEM)
Direct Substitute
Gibco™ Sodium Pyruvate (100 mM)
Add to KCell DMEM as an alternative to the specified medium. Not validated by Livestock Labs.
Antibiotic
Penicillin-Streptomycin
Protocol Specified
Gibco™ Penicillin-Streptomycin
Buffer
DPBS (no calcium, no magnesium)
Protocol Specified
Gibco™ DPBS, no calcium, no magnesium
Dissociation
Trypsin-EDTA (0.25%)
Protocol Specified
Gibco™ Trypsin-EDTA (0.25%), with phenol red
Cryoprotectant
CryoStor CS10
Protocol Specified
BioLife Solutions CryoStor® CS10
DMSO
Protocol Specified
Sigma-Aldrich Dimethyl Sulfoxide (DMSO)
Suppliers usually provide their full protocol, with volumes and usage instructions, after purchase. Ask us if you need details before ordering →
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Direct Substitute: assessed by Cellbase's technical team as equivalent for the same job.
Check specifications with each supplier before ordering.
What published studies used with these cells ShowHide Components that studies in Cellag Bio used with cells like these, and where to find them on Cellbase.Includes DMEM, DMEM/F12.
Based on 4 Cellag Bio studies · No proliferation studies on this species and cell type yet. Closest evidence: the same cell type in other species.
Basal medium
DMEMUsed in 3 of 4 Cellag Bio studies
Gibco™ DMEM, high glucose
DMEM/F12Used in 1 of 4 Cellag Bio studies
Gibco™ DMEM/F-12
Supplements
Also used in these studies, not currently on Cellbase: Alpha-ketoglutarate, Galactose, Glucose, Glutamate. Ask us to source these →
Based on 2 Cellag Bio studies · Limited evidence
Basal medium
DMEMUsed in 1 of 2 Cellag Bio studies
Gibco™ DMEM, high glucose
Supplements
Also used in these studies, not currently on Cellbase: Doxycycline. Ask us to source this →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Livestock Labs to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Livestock Labs with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Livestock Labs to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
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- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
A robust dermis-derived porcine fibroblast cell line isolated post-mortem (post-rigor) from a 6-month-old male pig sourced from an FSIS-inspected, USDA-regulated meat-processing facility in the United States. Donor animals were raised under an IACUC-approved protocol within an AAALAC-accredited program following Agriculture Guide standards, and tissues were collected and processed under institutional biosafety protocols into primary cells before cryopreservation.
The line was subsequently engineered via HDR-mediated targeted cassette integration, incorporating an immortalization factor and three pro-proliferative factors to enable long-term expansion and sustained growth performance. Cells are supplied frozen in a 1 mL cryovial (store in the vapor phase of liquid nitrogen; rapid thaw at 37°C recommended) and include a one-year research license.
A comprehensive documentation pack is provided, including donor and sourcing summary, characterization data, morphology images, long-term culture identity (karyotype and transcriptome PCA), recommended culture media, and storage/growth protocols, along with technical support for media optimization and process development; the license also includes ongoing media optimization updates and protocol refinements at no additional subscription cost.
For research use only, not for human or animal therapeutic use, diagnostic use, or consumption.
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Storage temperature
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Validation status
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Cold Chain Class
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Temperature Max (°C)
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Temperature Min (°C)
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Authentication method
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Cell count per vial
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Anchorage TypeAdherent
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Passage Range
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Primary vs immortalized
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Species
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Tissue type
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Mycoplasma Status
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Basal Medium
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Serum Type
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CO2 Percent
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Biosafety Level
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GMO Status
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Breed
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Species Detail
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Donor Age
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Donor Sex
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Doubling Time (hours)
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Pathogen Screening
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Donor Health Status
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Recommended Seeding Density Min (cells/cm²)
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Recommended Seeding Density Max (cells/cm²)
Published research
Studies on the same species and cell type in Cellag Bio.
- A simplified and defined serum-free medium for cultivating fat across species
- Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts
- Non-ammoniagenic proliferation and differentiation media for cultivated adipose tissue
- Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
- Chicken Embryo Fibroblast Viability and Trans-Differentiation Potential for Cultured Meat Production Across Passages
Studies from Cellag Bio, a database of published cultivated meat research.
Checkout Options
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Your supplier ships your order and invoices you directly for the goods.
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