Non-Animal Scaffolds Under Heat and pH Stress
For cultivated meat R&D teams, I assess scaffold stability by retained function - not appearance. Test your formulation through its actual heating, cooling and pH sequence. For adherent culture, check...
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Quest | SKU:
Quest Water Buffalo Primary Cell Lines - Myosatellite
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£1,000.00
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Supplier protocol
What the supplier's protocol for these cells calls for, and where to find it on Cellbase.
Basal medium
DMEM, high glucose
Protocol Specified
Corning® DMEM High Glucose
Serum
FBS
Protocol Specified
Sigma-Aldrich Fetal Bovine Serum
Growth factors
FGF2 (bovine, animal-free)
Protocol Specified
Bovine FGF-basic (FGF-2/bFGF), Animal-Free Recombinant Protein, PeproTech®
Protocol Specified
Protevo Bio bFGF2 – Bovine Fibroblast Growth Factor 2
Protocol Specified
Qkine Recombinant Bovine/Porcine FGF-2 (145 Aa) Protein
Buffer
PBS (without Ca and Mg)
Protocol Specified
Corning® Phosphate-Buffered Saline (PBS)
Dissociation
TrypLE Select
Protocol Specified
Gibco™ TrypLE™ Select Enzyme (1X), no phenol red
Cryoprotectant
DMSO
Protocol Specified
Sigma-Aldrich Dimethyl Sulfoxide (DMSO)
Attachment and coating
Laminin-511 E8 substrate
Protocol Specified
Merck Millipore ECMatrix™-511 E8 Laminin Substrate
Suppliers usually provide their full protocol, with volumes and usage instructions, after purchase. Ask us if you need details before ordering →
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with these cells ShowHide Components that studies in Cellag Bio used with cells like these, and where to find them on Cellbase.Includes DMEM, DMEM/F12, Ham's F-10, L-15.
Based on 52 Cellag Bio studies · No proliferation studies on this species and cell type yet. Closest evidence: the same cell type in other species.
Basal medium
DMEMUsed in 19 of 52 Cellag Bio studies
Gibco™ DMEM, high glucose
DMEM/F12Used in 19 of 52 Cellag Bio studies
Gibco™ DMEM/F-12
Ham's F-10Used in 12 of 52 Cellag Bio studies
Gibco™ Ham's F-10 Nutrient Mix
L-15Used in 2 of 52 Cellag Bio studies
Gibco™ Leibovitz's L-15 Medium
Other options (1)
KCell Leibovitz’s L-15
Serum or serum alternative
Also used in these studies, not currently on Cellbase: Albumin, Alfalfa protein isolate, Fetuin, Mung bean protein isolate. Ask us to source these →
Based on 53 Cellag Bio studies · No differentiation studies on this species and cell type yet. Closest evidence: the same cell type in other species.
Basal medium
DMEMUsed in 32 of 53 Cellag Bio studies
Gibco™ DMEM, high glucose
DMEM/F12Used in 4 of 53 Cellag Bio studies
Gibco™ DMEM/F-12
L-15Used in 3 of 53 Cellag Bio studies
Gibco™ Leibovitz's L-15 Medium
Other options (1)
KCell Leibovitz’s L-15
Ham's F-10Used in 2 of 53 Cellag Bio studies
Gibco™ Ham's F-10 Nutrient Mix
Supplements
InsulinUsed in 4 of 53 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Dexamethasone, IBMX, Oleic acid. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Quest to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Quest with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Quest to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
- Card (secure processing, immediate fulfilment)
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- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Quest Water Buffalo Myosatellite cell lines extend cultivated meat research into water buffalo as an alternative bovine species widely used in Asian and dairy markets. These primary muscle stem cells are derived from water buffalo muscle tissue and are supplied at passage one with full donor documentation. They enable comparative studies of growth, differentiation, and nutritional characteristics specific to myosatellite cell behavior across species.
The myosatellite cells are adherent and maintain viability above seventy percent after cryopreservation. These muscle-specific stem cells have been expanded successfully beyond twenty passages without loss of morphology or differentiation potential. Each vial contains one million viable cells that are certified free from bacteria, fungi, and mycoplasma. A complete protocol pack is included, covering thawing, seeding, passaging, and differentiation steps tailored for myosatellite culture.
Quest water buffalo myosatellite cell lines are offered under two license models. The research license covers non-commercial research and academic use. The commercial license permits use in commercial R&D and product development. Both include technical support and access to updated culture information.
These products are intended for research use only. They are not approved for human or diagnostic applications.
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Regulatory complianceISO 9001, GMP
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Scale compatibilityLab scale
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Storage temperature
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Cold Chain Class
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Temperature Max (°C)
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Temperature Min (°C)
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Authentication method-
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Cell count per vial
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Cell line name
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Cryopreservation viability (%)
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Differentiation capacity
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Anchorage TypeAdherent
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Origin country
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Passage Range
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Primary vs immortalized
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Species
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Tissue type
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Viability (%)
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Mycoplasma Status
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Basal Medium
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CO2 Percent
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Biosafety Level
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GMO Status
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License TypeR&D
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COA File
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Species Detail
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Doubling Time (hours)
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Tissue Collection Method
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Sterility Test Status
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Viral Screening Status
Published research
Cellag Bio has no studies on this species and cell type yet. Closest evidence: the same cell type in other species.
- iSsus3744: A Genome-Scale Model-Guided Strategy for Rational Media Design for Cultivated Pork
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
- Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells
Studies from Cellag Bio, a database of published cultivated meat research.
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Your supplier ships your order and invoices you directly for the goods.
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