Non-Animal Scaffolds Under Heat and pH Stress
For cultivated meat R&D teams, I assess scaffold stability by retained function - not appearance. Test your formulation through its actual heating, cooling and pH sequence. For adherent culture, check...
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Defined Bioscience | SKU:
Defined Bioscience FGF2-G3 Growth Factor (Bovine)
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£68.00
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
FGF2 (heat stable) · Proliferation
Protocol Specified
Cellcraft Spontaneously Immortalized Porcine Preadipocyte Cells
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with FGF2-G3 ShowHide Components that studies in Cellag Bio used alongside FGF2-G3, and where to find them on Cellbase. Limited evidenceIncludes DMEM/F12, Insulin.
Avian · Muscle cells (1)Bovine · Muscle cells (1)Fish and seafood · Muscle cells (1)Porcine · Muscle cells (1)
DMEM/F12Used in 1 of 1 Cellag Bio study
Defined Bioscience DMEM/F12 Basal Medium
InsulinUsed in 1 of 1 Cellag Bio study
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Albumin, Corn starch, DL-Alanine, Methylcellulose. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Defined Bioscience to research and production facilities.
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Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Defined Bioscience to ensure successful implementation.
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Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
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Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Defined Bioscience’s FGF2-G3 (Bovine) is a thermostabilised recombinant version of bovine fibroblast growth factor 2 engineered for use in cultivated-meat cell culture. It supports proliferation and maintenance of bovine muscle and stem cells with minimal medium supplementation. The enhanced thermostability of this variant ensures consistent activity for extended periods at 37 °C, eliminating the need for daily or weekend media changes and reducing process variability in long-term culture.
The protein sequence is derived from Bos taurus FGF2 (UniProt P03969) with nine key amino-acid substitutions introduced to improve thermostability and bioactivity. These modifications mirror those proven effective in the human FGF2-G3 variant, resulting in identical stability benefits. FGF2-G3 (Bovine) is recombinantly expressed in E. coli, animal-origin-free, and purified to greater than 95 % purity as confirmed by SDS-PAGE. It exhibits an EC₅₀ below 1 ng/mL in NIH-3T3 proliferation assays, indicating high potency at reduced concentrations compared with native FGF2.
Supplied as either a sterile 1 mg/mL liquid or lyophilized powder, the protein can be reconstituted into standard buffer formulations and substituted directly for wild-type FGF2 in serum-free and chemically defined media. Its species-specific sequence makes it ideal for bovine cell lines used in cultivated-beef development and scale-up research.
For research and development use only. Not for human or diagnostic applications.
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Scale compatibilityProduction scale
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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After mix stability (max days)
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After mix stability (min days)
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Animal origin freeYes
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Buffer additives allowedYes
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Buffer system
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Chemically definedYes
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Concentration
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Do not refilterYes
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Glucose (mg/L)
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Mix immediately after thawYes
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Optimized for
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pH Max
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pH Min
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Post‑thaw stability (max days)
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Post‑thaw stability (min days)
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Serum content
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Sterile filter pore size (µm)
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Xeno‑freeYes
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Appearance
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Purity (%)
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For Research Use OnlyYes
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Temperature Max (°C)
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Temperature Min (°C)
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Species
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Cell Line SpecificityAvian, Bovine, Seafood, Porcine
Published research using FGF2-G3
Used in 1 of 157 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
Studies from Cellag Bio, a database of published cultivated meat research.
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