Non-Animal Scaffolds Under Heat and pH Stress
For cultivated meat R&D teams, I assess scaffold stability by retained function - not appearance. Test your formulation through its actual heating, cooling and pH sequence. For adherent culture, check...
1
of
3
Loading...
Defined Bioscience | SKU:
Defined Bioscience HiDef® B8 Stem Cell Growth Medium
Regular price
£113.00
Unit price
/
Unavailable
(Plus Shipping - Calculated in Cart)
What published studies used with B8
Components that studies in Cellag Bio used alongside B8, and where to find them on Cellbase. Limited evidence
DMEM/F12Used in 1 of 1 Cellag Bio study
Defined Bioscience DMEM/F12 Basal Medium
VitronectinUsed in 1 of 1 Cellag Bio study
Qkine Recombinant Bovine Vitronectin Protein
Other options (1)
Qkine Recombinant Porcine Vitronectin Protein
Also used in these studies, not currently on Cellbase: Vibrio natriegens lysate. Ask us to source this →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Defined Bioscience to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Defined Bioscience with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Defined Bioscience to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
- Card (secure processing, immediate fulfilment)
- Bank transfer (proforma invoice issued at checkout)
- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Defined Bioscience’s HiDef® B8 Stem Cell Growth Medium is a complete, chemically defined formulation designed for feeder-free maintenance and expansion of human pluripotent stem cells. It provides a reliable foundation for cultivated-meat research by supporting long-term proliferation of mammalian stem and progenitor cells under serum-free conditions. The medium maintains cells in an undifferentiated state while reducing labor demands through a flexible feeding schedule that enables weekend-free culture.
HiDef B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and a thermostable variant of FGF2. This combination eliminates the need for additional supplementation while maintaining genomic stability and morphology for over fifty passages. The formulation has been validated across multiple human iPSC lines and is compatible with a wide range of matrices including Matrigel®, Geltrex™, and vitronectin substrates.
Available as a complete medium kit or as a supplement-only format for use with DMEM/F12 basal media, HiDef B8 offers full flexibility for process development. Once prepared aseptically, the complete medium can be stored at 2–8 °C for two weeks and supports consistent growth without karyotypic drift.
For research and development use only. Not for human or diagnostic applications.
-
Regulatory compliance------
-
Scale compatibilityProduction scale
-
Shelf life (months)
-
Storage temperature
-
Validation status
-
Product Sterilisation Method
-
Cold Chain Class
-
After mix stability (max days)
-
Aliquoting recommendedYes
-
Animal origin freeYes
-
Buffer additives allowedYes
-
Chemically definedYes
-
Complete media storage temperature
-
Concentration
-
Do not refilterYes
-
Endotoxin level (EU/mL)
-
Media categorySupplementSupplementSupplementSupplementSupplementSupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, Supplement
-
Mix immediately after thawYes
-
Non‑CO₂ Buffer Option
-
Optimized for
-
pH Max
-
pH Min
-
Post‑thaw stability (max days)
-
Serum content
-
Sterile filter pore size (µm)
-
Xeno‑freeYes
-
Media FormLiquid
-
Light SensitivityYes
-
Temperature Max (°C)
-
Temperature Min (°C)
-
Cell Line SpecificityAvian, Bovine, Seafood, Porcine
Published research using B8
Used in 1 of 157 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
Studies from Cellag Bio, a database of published cultivated meat research.
Checkout Options
Card
Secure payment processing. Card details are not stored.
Bank Transfer or Purchase Order
Select bank transfer at checkout to receive a proforma invoice. Purchase orders accepted. Your order processes upon payment receipt.
Your supplier ships your order and invoices you directly for the goods.
Shop
Insights & News
View all-
-
LCA Data for Cultivated Meat Equipment Selection
For cultivated meat bioprocess engineers, I compare equipment impacts per 1 kg of usable finished product - not by rated power. I first compare bioreactor systems for process fit, food...
-
Serum-Free Media Design for Myoblast Cell Lines
For myoblast culture teams, I would select a serum-free medium by two outcomes: viable cell expansion and retained myotube-forming capacity. Start with your cell line’s history and measurable acceptance limits...
-
Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
-
UCOE vs cHS4 for Stable Gene Expression
For cultivated meat R&D teams, I would choose UCOE or cHS4 by measured expression retention - not initial expression strength. Compare matched constructs with an unprotected control across independent clones....
-
Spent Media Regeneration: Batch vs Continuous Routes
If you run cultivated meat process development, the choice is simple in principle: batch gives tighter lot isolation; continuous cuts hold time and smooths media return - but only if...
-
Gene Knockout Design for Cultivated Meat Cell Lines
If a knockout does not improve a defined process trait, it is just an edit, not a usable cell line. For bioprocess engineers, cell culture scientists, and cultivated meat R&D...
-
TEA vs LCA in Waste System Uncertainty
If you use TEA and LCA to judge the same cultivated meat waste route, you should expect different answers. That is not a modelling failure. It happens because TEA tests...
