Customising Chassis Cells for Structured Meat Products
For cultivated meat R&D teams, producing structured whole-cuts like steaks or fillets requires more than just growing cells. The key lies in chassis cells - muscle, fat, and connective tissue...
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Defined Bioscience | SKU:
Defined Bioscience HiDef® B8 Stem Cell Growth Medium
Regular price
£112.00
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Availability
In stock. Ships globally direct from Defined Bioscience to research and production facilities.
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Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Defined Bioscience to ensure successful implementation.
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Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Defined Bioscience’s HiDef® B8 Stem Cell Growth Medium is a complete, chemically defined formulation designed for feeder-free maintenance and expansion of human pluripotent stem cells. It provides a reliable foundation for cultivated-meat research by supporting long-term proliferation of mammalian stem and progenitor cells under serum-free conditions. The medium maintains cells in an undifferentiated state while reducing labour demands through a flexible feeding schedule that enables weekend-free culture.
HiDef B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and a thermostable variant of FGF2. This combination eliminates the need for additional supplementation while maintaining genomic stability and morphology for over fifty passages. The formulation has been validated across multiple human iPSC lines and is compatible with a wide range of matrices including Matrigel®, Geltrex™, and vitronectin substrates.
Available as a complete medium kit or as a supplement-only format for use with DMEM/F12 basal media, HiDef B8 offers full flexibility for process development. Once prepared aseptically, the complete medium can be stored at 2–8 °C for two weeks and supports consistent growth without karyotypic drift.
For research and development use only. Not for human or diagnostic applications.
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Regulatory compliance------
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Scale compatibilityProduction scale
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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After mix stability (max days)
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Aliquoting recommendedYes
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Animal origin freeYes
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Buffer additives allowedYes
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Chemically definedYes
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Concentration
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Do not freezeYes
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Endotoxin level (EU/mL)
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Media categorySupplementSupplementSupplementSupplementSupplementSupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, Supplement
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Mix immediately after thawYes
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Non‑CO₂ Buffer Option
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Optimized for
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pH Max
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pH Min
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Post‑thaw stability (max days)
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Serum content
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Sterile filter pore size (µm)
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Xeno‑freeYes
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Media FormLiquid
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Light SensitivityYes
Cellag Bio is the structured intelligence layer for cultivated meat R&D — scattered published research distilled into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before committing budget to a medium, reagent, or cell line, check the evidence behind it: real experimental outcomes and failure modes, not marketing claims.
Search by media, reagent, cell line or species (e.g. "Beefy-9" or "B8") to find the records that use it.
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