Non-Animal Scaffolds Under Heat and pH Stress
For cultivated meat R&D teams, I assess scaffold stability by retained function - not appearance. Test your formulation through its actual heating, cooling and pH sequence. For adherent culture, check...
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Defined Bioscience | SKU:
Defined Bioscience FGF2 Growth Factor (Tuna)
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What published studies used with FGF2
Components that studies in Cellag Bio used alongside FGF2, and where to find them on Cellbase.
Bovine · Muscle cells (24)Porcine · Muscle cells (7)Bovine · Pluripotent stem cells (6)Avian · Muscle cells (4)
FBSUsed in 38 of 54 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
DMEMUsed in 30 of 54 Cellag Bio studies
KCell DMEM
DMEM/F12Used in 23 of 54 Cellag Bio studies
Defined Bioscience DMEM/F12 Basal Medium
Horse serumUsed in 12 of 54 Cellag Bio studies
Gibco™ Horse Serum, New Zealand Origin
Also used in these studies, not currently on Cellbase: Matrigel, Gelatin. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Defined Bioscience to ensure successful implementation.
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Defined Bioscience’s FGF2 (Tuna) is a recombinant, animal-origin-free fibroblast growth factor 2 derived from Thunnus albacares (yellowfin tuna). It supports cell proliferation, differentiation, and maintenance in serum-free and chemically defined media, particularly for marine and aquatic cell lines used in cultivated-seafood research. As a native fish homolog, it enhances compatibility with piscine cells while maintaining cross-species functionality across mammalian and avian models.
Produced recombinantly in E. coli, FGF2 (Tuna) is supplied carrier-free and purified to greater than 95 % by SDS-PAGE, ensuring consistent purity and performance. The protein contains an N-terminal hexahistidine tag and protease cleavage site and is formulated in phosphate buffer at 1 mg/mL. Biological activity testing demonstrates an EC₅₀ below 2 ng/mL in NIH-3T3 proliferation assays, confirming high potency at low concentrations.
The recombinant production process is fully animal-component-free and supports reproducible performance across long-term culture conditions. FGF2 (Tuna) can be incorporated directly into basal or serum-free media formulations to improve cell viability and proliferation in fish cell lines, aiding optimization of cultivated-seafood workflows from R&D through to pilot-scale studies.
For research and development use only. Not for human or diagnostic applications.
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Scale compatibilityProduction scale
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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After mix stability (max days)
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After mix stability (min days)
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Animal origin freeYes
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Buffer additives allowedYes
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Buffer system
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Chemically definedYes
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Concentration
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Do not refilterYes
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Glucose (mg/L)
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Mix immediately after thawYes
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Optimized for
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pH Max
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pH Min
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Post‑thaw stability (max days)
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Post‑thaw stability (min days)
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Serum content
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Sterile filter pore size (µm)
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Xeno‑freeYes
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Appearance
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Purity (%)
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For Research Use OnlyYes
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Temperature Max (°C)
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Species
Published research using FGF2
Used in 54 of 157 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- Tissue-like cultured fish fillets through a synthetic food pipeline
- Establishment and Characterization of Continuous Satellite Muscle Cells from Olive Flounder (Paralichthys olivaceus): Isolation, Culture Conditions, and Myogenic Protein Expression
- Characterization of Proliferation Medium and Its Effect on Differentiation of Muscle Satellite Cells from Larimichthys crocea in Cultured Fish Meat Production
- Fish Pluripotent Stem-Like Cell Line Induced by Small-Molecule Compounds From Caudal Fin and its Developmental Potentiality
- Advances in development of long-term embryonic stem cell-like cultures from a marine fish, Sciaenops ocellatus
Studies from Cellag Bio, a database of published cultivated meat research.
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