Qkine Recombinant Zebrafish FGF-2 Protein

Qkine's food grade zebrafish FGF-2 is a species-matched fibroblast growth factor essential for maintaining stem cell proliferation, self-renewal, and myoblast expansion in cultivated meat cell culture. Species-matched growth factors deliver superior receptor binding efficiency compared to heterologous proteins, enabling lower supplementation concentrations, improved cellular responses, and enhanced cost-effectiveness in serum-free media formulations for zebrafish cell lines.

This food-grade recombinant protein is produced in E. coli expression systems and purified to >98% purity by SDS-PAGE quantitative densitometry. Manufactured in the UK under a HACCP food manufacturing regime, the protein is animal-origin-free (AOF), carrier protein-free, and tag-free to ensure exceptional purity and lot-to-lot consistency suitable for food applications. Comprehensive quality control includes mass spectrometry confirmation of expected molecular weight, recovery testing from stock vials (>95%), endotoxin analysis (<0.05 EU/μg protein), and full traceability documentation including certificates of analysis, certificates of origin, allergen analysis, beta-lactam-free certification, and animal-origin-free verification.

This protein is supplied lyophilized and should be reconstituted in 10 mM HCl or phosphate buffer at concentrations above 50 µg/mL, with optional addition of carrier protein for enhanced stability. Single-use aliquots are recommended to maintain protein integrity, with storage at -20°C for short-term use or -80°C for long-term storage. Suitable for cultivated meat media optimization, process development, commercial-scale production, and regulatory submissions. This food-grade protein is manufactured to support novel food dossier preparation, regulatory approval processes, and commercial manufacturing under GMP-equivalent standards.