DMEM vs F12 for Cultivated Meat Cell Lines
For cultivated meat R&D teams, I’d screen DMEM, Ham’s F12 and a 1:1 DMEM/F12 mix before choosing a basal medium. None is complete on its own: your cell line, supplements...
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Corning | SKU:
15-012-CV
Corning® MEM Alpha Medium
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
MEM Alpha, low glucose · Proliferation
Protocol Specified
Quest Porcine Primary Cell Lines - Adipose Stem Cell
Quest Water Buffalo Primary Cell Lines - Adipose
Quest Bovine Primary Cell Lines - British Blue Adipose Stem Cell
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with α-MEM ShowHide Components that studies in Cellag Bio used alongside α-MEM, and where to find them on Cellbase.Includes FBS, FGF2, DMEM, Activin A.
FBSUsed in 5 of 5 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
FGF2Used in 3 of 5 Cellag Bio studies
Qkine Recombinant Eel FGF-2 (145 Aa) Protein
DMEMUsed in 2 of 5 Cellag Bio studies
Gibco™ DMEM, high glucose
Activin AUsed in 1 of 5 Cellag Bio studies
Qkine Recombinant Bovine/Porcine Activin a Protein
Other options (1)
CultureGro™ Human Activin-A Animal-Free Recombinant Protein
BMP4Used in 1 of 5 Cellag Bio studies
CultureGro™ Human BMP-4 Animal Free Recombinant Protein
Also used in these studies, not currently on Cellbase: Ascorbic acid. Ask us to source this →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Corning to ensure successful implementation.
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Corning® MEM (Minimum Essential Medium) Alpha Medium is an advanced modification of the traditional MEM, designed to support the propagation of mammalian fibroblasts and a variety of other cell subtypes. Compared to Basal Medium Eagle (BME), MEM Alpha contains significantly higher concentrations of amino acids to more closely mimic the natural protein composition of mammalian cells, promoting robust cell health and attachment.
The 15-012-CV formulation includes Earle’s salts for effective buffering but is provided without ribonucleosides, deoxyribonucleosides, or L-glutamine. This allows researchers the flexibility to customize the medium with specific supplements required for specialized cell lines or selection assays. Manufactured in ISO 13485-certified facilities, this medium guarantees the high purity and quality standards essential for modern biotechnology.
Supplied as a 500 mL sterile liquid, Corning® MEM Alpha is a foundational tool for laboratories involved in genomics, stem cell research, and in vitro fertilization (IVF). Its reliable performance ensures a stable environment for maintaining cell monolayers and scaling up critical research processes.
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Regulatory complianceISO 13485
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Storage temperature
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Cold Chain Class
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Animal origin freeYes
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Buffer system
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Chemically definedYes
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Concentration
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Glucose (mg/L)
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Phenol redYes
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Serum content
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Media FormLiquid
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Sodium PyruvateYes
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Temperature Max (°C)
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Temperature Min (°C)
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Basal Medium
Published research using α-MEM
Used in 5 of 157 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- Scale-up of an intensified bioprocess for the expansion of bovine adipose-derived stem cells (bASCs) in stirred tank bioreactors
- Establishment of porcine embryonic stem cells in simplified serum free media and feeder free expansion
- Edible electrospun materials for scalable cultivated beef production
- Cultured meat platform developed through the structuring of edible microcarrier-derived microtissues with oleogel-based fat substitute
- Bovine Fibroblast-Derived Extracellular Matrix Promotes the Growth and Preserves the Stemness of Bovine Stromal Cells during In Vitro Expansion
Studies from Cellag Bio, a database of published cultivated meat research.
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