DMEM vs F12 for Cultivated Meat Cell Lines
For cultivated meat R&D teams, I’d screen DMEM, Ham’s F12 and a 1:1 DMEM/F12 mix before choosing a basal medium. None is complete on its own: your cell line, supplements...
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Sigma-Aldrich | SKU:
D2650-100ML
Sigma-Aldrich Dimethyl Sulfoxide (DMSO)
Regular price
£200.00
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Unavailable
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
DMSO · Cryopreservation
Protocol Specified
Quest Bovine Primary Cell Lines - Aberdeen Angus Cross Myosatellite
Quest Bovine Primary Cell Lines - Aberdeen Angus Pure Myosatellite
Quest Bovine Primary Cell Lines - Limousine Myosatellite
Other cell lines (6)
Quest Porcine Primary Cell Lines - Myosatellite
Quest Water Buffalo Primary Cell Lines - Myosatellite
Quest Bovine Primary Cell Lines - British Blue Myosatellite
Quest Water Buffalo Primary Cell Lines - Adipose
Livestock Labs Porcine Fibroblasts
Quest Bovine Primary Cell Lines - British Blue Adipose Stem Cell
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
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Sigma-Aldrich Dimethyl Sulfoxide (DMSO), Hybri-Max™ grade, is a sterile-filtered cryoprotectant with ≥99.7% purity. This polar aprotic solvent is widely used in cell culture for the cryopreservation of cells, tissues, and organs, as well as in molecular biology applications such as PCR.
This formulation (Cat. No. D2650) is specially tested for hybridoma applications and evaluated for suitability in cell freezing. It is sterile-filtered through a 0.2 μm membrane and tested for endotoxin and water content (≤0.1%), ensuring consistent performance in sensitive cell culture systems. The product is supplied in amber glass bottles to protect from light.
As a cryoprotectant, DMSO protects cells from mechanical damage caused by ice crystal formation during freezing. It is commonly used in cell freezing media for primary cells, secondary cultures, recombinant cell lines, aneuploid and hybridoma cell lines, embryonic stem cells (ESCs), and hematopoietic stem cells, often in combination with serum or serum albumin.
D2650 is a liquid at room temperature but may solidify if stored in cool conditions. If solidified, warm to room temperature to re-liquefy; product quality is not affected. Store at room temperature in a dry environment. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Sterile filter pore size (µm)
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Media FormLiquid
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For Research Use OnlyYes
Cellag Bio has no published studies on this product yet.
Cellag Bio is a database of published cultivated meat research, organized into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before you commit budget to a medium, reagent, or cell line, check the evidence behind it.
Search by medium, reagent, cell line, or species (for example "Beefy-9" or "B8") to find the studies that use it.
Search the Cellag Bio database →
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