Worlds First Cultivated Meat B2B Marketplace: Read Announcement
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ECMatrix-511 E8 Laminin Substrate is a xeno-free, ready-to-use recombinant laminin-511 E8 fragment designed for feeder-free culture of human pluripotent stem cells (ES and iPS cells). It supports stable maintenance and efficient expansion by binding to the α6β1 integrin expressed on pluripotent stem cells, providing a defined and animal-free surface for consistent cell culture.
This formulation (Cat. No. CC160100ML) is produced in CHO-S cells under serum-free conditions, ensuring consistent, animal-free performance. It is supplied as a ready-to-use liquid at 1.6 μg/mL in recombinant human serum albumin (HSA) and phosphate-buffered saline (PBS), eliminating the need for plate precoating. The substrate undergoes rigorous quality control, including purity (>95% by SDS-PAGE), endotoxin (≤750 EU/mg), sterility, mycoplasma testing, and integrin binding affinity confirmation (10 nM).
Key features include a xeno-free and animal-free format with lot-to-lot consistency; no plate precoating required, saving time during passaging; support for single-cell passaging without ROCK inhibitor, enabling CRISPR editing or clonal isolation; higher adhesion and growth rates; and easy handling with no chilling of consumables required.
Store at 2–8°C. Protect from light and avoid multiple freeze-thaw cycles. For coating, dilute to 0.25 μg/cm² and incubate for 1 hour at 37°C, 3 hours at room temperature, or overnight at 4°C. Remove remaining fluid before use; do not rinse. Detach cells into small clumps using Accutase or a similar dissociation reagent. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
Cellag Bio is the structured intelligence layer for cultivated meat R&D — scattered published research distilled into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before committing budget to a medium, reagent, or cell line, check the evidence behind it: real experimental outcomes and failure modes, not marketing claims.
Search by media, reagent, cell line or species (e.g. "Beefy-9" or "B8") to find the records that use it.
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Regulatory compliance
Scale compatibility
Shelf life (months)
Storage temperature
Validation status
Variant structure
Product Sterilisation Method
After mix stability (max days)
After mix stability (min days)
Aliquoting recommended
Animal origin free
Buffer additives allowed
Buffer system
Chemically defined
Complete media storage temperature
Concentration
Contains animal components
Contains BSA
Do not freeze
Do not refilter
Endotoxin level (EU/mL)
Food grade status
Glucose (mg/L)
Media category
Mix immediately after thaw
Non‑CO₂ Buffer Option
Optimized for
Osmolality (mOsm/kg)
Osmolarity (mOsm/kg)
pH Max
pH Min
Phenol red
Post‑thaw stability (max days)
Post‑thaw stability (min days)
Refreezing allowed
Serum content
Sterile filter pore size (µm)
Xeno‑free
Media Form
L-glutamine
Sodium Pyruvate
HEPES
Appearance
CAS number
Chemical name
Chemical structure image
Common name
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Light Sensitivity
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Polymer–LAP Ratio
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DO control
Edible components
Harvest method
Material
Max Cell Density (cells/mL)
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Bioreactor Configurations
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Dimensions diameter (mm)
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Edibility status
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Kit composition
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Porosity (%)
Protein content (%)
Protein source
Quantity per kit
Scaffold form
Surface area (m²/g)
Tensile strength (kPa)
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Usage Guidance Available
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Lattice Parameters
Mechanical Targets Supported
Nutritional Targets Supported
Mechanical Target Specs
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Legume Source Flag
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Sedimentation Velocity
Swelling Ratio
Size Distribution (CV%)
Surface Charge
Surface Coating
Dissolution Trigger
Certified for food use
Customization options
Density (g/mL)
Material type
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Particle size distribution
Particle size (µm)
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Stiffness customizable
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Tested cell lines
Therapeutic grade available
Accuracy
Calibration frequency
Measurement range
Sterilization compatible
Parameter
Diameter mm
Process Connection
Electrical Connector
Output Sensor
Temperature Sensor Type
Wetted Parts
Measurement Principle
Optical Path Length mm
Wavelength nm
Electrolyte
Regulatory Approval
Measuring Range
Configurable Values
Conductivity Range mS/cm
Accuracy at 25°C
Precision at 25°C
Certification
Operating Temp Min °C
Operating Temp Max °C
Operating Voltage VDC
Pressure Range bar g
IP Rating
Autoclavable
Steam Sterilizable SIP
CIP Compatible
Single Use Option
Authentication method
Cell count per vial
Cell line name
Cryopreservation viability (%)
Differentiation capacity
Anchorage Type
Origin country
Passage Range
Primary vs immortalized
Species
Tissue type
Viability (%)
Immortalization Method
Mycoplasma Status
Culture Mode Detail
Basal Medium
Serum Type
Coating Required
Recommended Coating
CO2 Percent
Biosafety Level
GMO Status
MTA Required
License Type
COA File
Breed
Species Detail
Donor Age
Donor Sex
Food-Safe Status
Doubling Time
Karyotype Data Available
Pathogen Screening
Donor Health Status
Tissue Collection Method
Authentication Status
Last Authentication Date
Mycoplasma Test Method
Sterility Test Status
Viral Screening Status
Passage at Banking
Max Passages Before Plateau
Differentiation Validation Method
Marker Expression Confirmed
Recommended Seeding Density
Thawing Protocol Available
Passaging Protocol Available
Availability Status
Population Doubling Limit
Growth Plateau Indicator
PDL Before Plateau
Storage Format
Humidity Required
Spinner Flask Drive
Impeller Design
Side Port Count
Cap Type
Bottle Thread
Coating Method
Coating Stability
Mesh Count
Prefilter Included
Color Coding
Harvest Arm Present
