DMEM vs F12 for Cultivated Meat Cell Lines
For cultivated meat R&D teams, I’d screen DMEM, Ham’s F12 and a 1:1 DMEM/F12 mix before choosing a basal medium. None is complete on its own: your cell line, supplements...
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Sigma-Aldrich | SKU:
Sigma-Aldrich Fetal Bovine Serum
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
FBS · Proliferation
Protocol Specified
Quest Bovine Primary Cell Lines - Aberdeen Angus Cross Myosatellite
Quest Bovine Primary Cell Lines - Aberdeen Angus Pure Myosatellite
Quest Bovine Primary Cell Lines - Limousine Myosatellite
Other cell lines (6)
Quest Porcine Primary Cell Lines - Myosatellite
Quest Water Buffalo Primary Cell Lines - Myosatellite
Quest Bovine Primary Cell Lines - British Blue Myosatellite
Quest Bovine Primary Cell Lines - British Blue Adipose Stem Cell
Quest Porcine Primary Cell Lines - Adipose Stem Cell
Quest Water Buffalo Primary Cell Lines - Adipose
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with FBS ShowHide Components that studies in Cellag Bio used alongside FBS, and where to find them on Cellbase.Includes DMEM, FGF2, DMEM/F12, Horse serum.
Bovine · Muscle cells (35)Avian · Muscle cells (12)Bovine · Mesenchymal stem cells (12)Porcine · Muscle cells (11)
DMEMUsed in 76 of 119 Cellag Bio studies
Gibco™ DMEM, high glucose
FGF2Used in 37 of 119 Cellag Bio studies
Qkine Recombinant Eel FGF-2 (145 Aa) Protein
DMEM/F12Used in 32 of 119 Cellag Bio studies
Gibco™ DMEM/F-12
Horse serumUsed in 29 of 119 Cellag Bio studies
Gibco™ Horse Serum, New Zealand Origin
InsulinUsed in 27 of 119 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Matrigel. Ask us to source this →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Sigma-Aldrich Fetal Bovine Serum (FBS) is a sterile-filtered, non-USA origin serum suitable for a wide range of mammalian cell culture applications. FBS remains the most widely used undefined supplement in cell culture due to its ability to provide essential nutrients, growth factors, and protective functions that are difficult to replicate in serum-free systems.
This formulation (Cat. No. F7524) is sourced from non-USA origin and undergoes rigorous quality testing. It is sterile-filtered three times through 0.1 μm membranes and tested for endotoxin (≤10 EU/mL), hemoglobin (≤25 mg/dL), and the absence of bacteria, viruses, and mycoplasma.
FBS is commonly used as a supplement in classical media such as Minimum Essential Medium (MEM), Dulbecco's Modified Eagle Medium (DMEM), Iscove's Modified Eagles Medium (IMDM), M199, and RPMI-1640. It supports the growth and maintenance of a variety of cell lines, including primary cells, immortalized lines, and hybridomas.
Supplied as a liquid, this product is shipped on dry ice and should be stored at –20°C upon arrival. Thaw at 2–8°C or room temperature, and avoid repeated freeze-thaw cycles. For optimal performance, use in accordance with standard aseptic techniques. This product is not available for sale in Canada or the USA. For availability in other regions, please consult your local supplier. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Contains animal componentsYes
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Contains BSAYes
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Endotoxin level (EU/mL)
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Media FormLiquid
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Published research using FBS
Used in 119 of 167 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- iSsus3744: A Genome-Scale Model-Guided Strategy for Rational Media Design for Cultivated Pork
- Engineered marble-like bovine fat tissue for cultured meat
- Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
- Decellularized spinach: An edible scaffold for laboratory-grown meat
- Scale-up of an intensified bioprocess for the expansion of bovine adipose-derived stem cells (bASCs) in stirred tank bioreactors
Studies from Cellag Bio, a database of published cultivated meat research.
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