DMEM vs F12 for Cultivated Meat Cell Lines
For cultivated meat R&D teams, I’d screen DMEM, Ham’s F12 and a 1:1 DMEM/F12 mix before choosing a basal medium. None is complete on its own: your cell line, supplements...
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Gibco | SKU:
11330032
Gibco™ DMEM/F-12, HEPES
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
DMEM/F-12, HEPES · Proliferation
Protocol Specified
Opo Bio Bovine Angus Satellite Cells
Opo Bio Bovine Angus Fibroblasts
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with DMEM/F12 ShowHide Components that studies in Cellag Bio used alongside DMEM/F12, and where to find them on Cellbase.Includes FBS, FGF2, Insulin, DMEM.
Bovine · Muscle cells (18)Bovine · Pluripotent stem cells (6)Porcine · Muscle cells (6)Avian · Muscle cells (5)
FBSUsed in 30 of 51 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
FGF2Used in 23 of 51 Cellag Bio studies
Qkine Recombinant Eel FGF-2 (145 Aa) Protein
InsulinUsed in 15 of 51 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
DMEMUsed in 14 of 51 Cellag Bio studies
Gibco™ DMEM, high glucose
Also used in these studies, not currently on Cellbase: Matrigel, Dexamethasone. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Gibco™ DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium designed to support the growth of a broad range of mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. This 1:1 mixture combines the high concentrations of glucose, amino acids, and vitamins from DMEM with the wide variety of components found in Ham’s F-12.
This specific formulation is modified with L-glutamine, HEPES, and phenol red. The addition of HEPES provides effective buffering to ensure pH stability, while the sodium bicarbonate system requires a 5–10% CO₂ environment to maintain physiological conditions. Produced in cGMP-compliant facilities, Gibco™ DMEM/F-12 contains no proteins, lipids, or growth factors.
Each batch is manufactured to meet rigorous quality standards for sterility and performance, making this medium an ideal foundation for serum-free media systems or custom supplementations (commonly 10% fetal bovine serum).
This medium is stable for up to 12 months when stored at 2–8°C and protected from light. It is shipped at ambient temperature and is suitable for both research use and further manufacturing applications. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Regulatory compliancecGMP, ISO 13485
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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Animal origin freeYes
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Concentration
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Glucose (mg/L)
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Phenol redYes
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L-glutamineYes
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HEPESYes
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Appearance
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Temperature Max (°C)
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Temperature Min (°C)
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Basal Medium
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CO2 Percent
Published research using DMEM/F12
Used in 51 of 157 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- iSsus3744: A Genome-Scale Model-Guided Strategy for Rational Media Design for Cultivated Pork
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
- Non-ammoniagenic proliferation and differentiation media for cultivated adipose tissue
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
Studies from Cellag Bio, a database of published cultivated meat research.
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