DMEM vs F12 for Cultivated Meat Cell Lines
For cultivated meat R&D teams, I’d screen DMEM, Ham’s F12 and a 1:1 DMEM/F12 mix before choosing a basal medium. None is complete on its own: your cell line, supplements...
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Gibco | SKU:
11039021
Gibco™ DMEM/F-12, HEPES, no phenol red
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What published studies used with DMEM/F12
Components that studies in Cellag Bio used alongside DMEM/F12, and where to find them on Cellbase.
Bovine · Muscle cells (18)Porcine · Muscle cells (7)Bovine · Pluripotent stem cells (6)Avian · Muscle cells (5)
FBSUsed in 32 of 53 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
FGF2Used in 23 of 53 Cellag Bio studies
Qkine Recombinant Eel FGF-2 (145 Aa) Protein
InsulinUsed in 16 of 53 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
DMEMUsed in 15 of 53 Cellag Bio studies
Gibco™ DMEM, high glucose
Also used in these studies, not currently on Cellbase: Matrigel, Dexamethasone. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Gibco™ DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium designed to support the growth of a broad range of mammalian cells. This 1:1 mixture combines the high concentrations of glucose, amino acids, and vitamins from DMEM with the wide variety of components found in Ham's F-12.
This specific formulation (Cat. No. 11039021) is modified with L-glutamine, HEPES, and does not contain phenol red. The addition of HEPES provides powerful pH buffering that minimizes fluctuations outside of a CO₂ incubator, making it ideal for applications requiring extended observation or manipulation. The absence of phenol red makes this medium suitable for assays or experiments where the pH indicator might interfere with measurements.
Produced in cGMP-compliant facilities, Gibco™ DMEM/F-12 contains no proteins, lipids, or growth factors. Each batch is manufactured to meet rigorous quality standards for sterility and performance.
This medium is stable for up to 12 months when stored at 2–8 °C and protected from light. It is shipped at ambient temperature and is suitable for both research use and further manufacturing applications.
For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Regulatory complianceISO 13485, cGMP
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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Animal origin freeYes
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Buffer system
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Chemically definedYes
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Concentration
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Do not freezeYes
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Glucose (mg/L)
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Media category
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Serum content
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Xeno‑freeYes
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Media FormLiquid
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L-glutamineYes
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Sodium PyruvateYes
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HEPESYes
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Temperature Max (°C)
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Temperature Min (°C)
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Basal Medium
Published research using DMEM/F12
Used in 53 of 167 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- iSsus3744: A Genome-Scale Model-Guided Strategy for Rational Media Design for Cultivated Pork
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
- Non-ammoniagenic proliferation and differentiation media for cultivated adipose tissue
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
Studies from Cellag Bio, a database of published cultivated meat research.
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