DMEM vs F12 for Cultivated Meat Cell Lines
For cultivated meat R&D teams, I’d screen DMEM, Ham’s F12 and a 1:1 DMEM/F12 mix before choosing a basal medium. None is complete on its own: your cell line, supplements...
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Gibco | SKU:
Gibco™ L-Glutamine (200 mM)
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
L-Glutamine · Proliferation
Protocol Specified
Quest Bovine Primary Cell Lines - British Blue Adipose Stem Cell
Quest Porcine Primary Cell Lines - Adipose Stem Cell
Quest Water Buffalo Primary Cell Lines - Adipose
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with L-glutamine ShowHide Components that studies in Cellag Bio used alongside L-glutamine, and where to find them on Cellbase.Includes FBS, DMEM/F12.
Bovine · Pluripotent stem cells (1)Ovine · Mesenchymal stem cells (1)Porcine · Mesenchymal stem cells (1)
FBSUsed in 3 of 3 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
DMEM/F12Used in 2 of 3 Cellag Bio studies
Gibco™ DMEM/F-12
Also used in these studies, not currently on Cellbase: 2-Mercaptoethanol, B27, CHIR99021, Dexamethasone. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Gibco to ensure successful implementation.
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Gibco™ L-Glutamine is an essential amino acid supplement used in cell culture to support optimal cell growth and proliferation. L‑glutamine serves as a key energy source and is required for protein and nucleic acid synthesis in most mammalian cells, making it a critical component of classical media formulations.
This formulation (Cat. No. 25030081) is supplied as a 200 mM (100X) concentrated, sterile-filtered liquid. It is recommended for addition to cell culture media at a final concentration of 2 mM. Produced in cGMP-compliant facilities, this supplement is manufactured under rigorous quality standards; each batch is tested for sterility, endotoxin levels, and biological activity to ensure consistent performance.
L‑glutamine is labile in solution and degrades over time, particularly at elevated temperatures and under light exposure. To maintain stability, it should be added to media immediately before use and used within two weeks when stored at 2–8°C.
This solution should be stored at –20°C and protected from light. After thawing, store at 2–8°C and use within one month. Avoid repeated freeze-thaw cycles. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Regulatory compliancecGMP
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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Animal origin freeYes
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Chemically definedYes
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Concentration
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Serum content
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Xeno‑freeYes
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Media FormLiquid
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L-glutamineYes
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Common name
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Light SensitivityYes
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Reagent category
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For Research Use OnlyYes
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Temperature Max (°C)
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Temperature Min (°C)
Published research using L-glutamine
Used in 3 of 157 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- LCDM medium supports the derivation of bovine extended pluripotent stem cells with embryonic and extraembryonic potency in bovine–mouse chimeras from iPSCs and bovine fetal fibroblasts
- Evaluation of food-grade antifoams for biocompatibility, differentiation, and bioprocess performance in porcine mesenchymal stem cells cultivation for cellular agriculture
- Isolation and characterization of ovine mesenchymal stem cells derived from peripheral blood
Studies from Cellag Bio, a database of published cultivated meat research.
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