Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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Gibco | SKU:
A5209501
Gibco™ FBS, Qualified, Heat Inactivated
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What published studies used with FBS
Components that studies in Cellag Bio used alongside FBS, and where to find them on Cellbase.
Bovine · Muscle cells (8)Avian · Muscle cells (4)Bovine · Fat cells (4)Bovine · Pluripotent stem cells (4)
DMEMUsed in 22 of 38 Cellag Bio studies
Gibco™ DMEM, high glucose
FGF2Used in 15 of 38 Cellag Bio studies
Qkine Recombinant Eel FGF-2 (145 Aa) Protein
DMEM/F12Used in 11 of 38 Cellag Bio studies
Gibco™ DMEM/F-12
Horse serumUsed in 8 of 38 Cellag Bio studies
Gibco™ Horse Serum, New Zealand Origin
InsulinUsed in 7 of 38 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Matrigel. Ask us to source this →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Gibco™ Fetal Bovine Serum, Value, Heat Inactivated is a fetal bovine serum designed to provide an excellent value for basic cell culture, specialty research, and specific assays. It is heat inactivated to inactivate complement, which can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Heat-inactivated serum is recommended for applications including immunological studies and the culture of embryonic stem cells, insect cells, and smooth muscle cells.
The serum is triple-filter sterilized using a 0.1 μm filter and is prepared using an aseptic process. Heat inactivation is performed at 56°C for 30 minutes, with mixing to promote heat distribution and reduce protein aggregation and flocculant precipitation. Heat inactivation can also help achieve bottle-to-bottle and lot-to-lot stability by neutralizing factors that can vary between lots.
Gibco™ Fetal Bovine Serum, Value, Heat Inactivated is supplied in a One Shot format and can be added to culture media according to the requirements of the application and cell line. Thermo Fisher states that FBS is commonly added to culture medium at 2–10%, with 10% used for most basal media, while some applications may require up to 20% supplementation.
This heat-inactivated serum is suitable for basic cell culture, specialty research, and specific assays where complement-inactivated FBS is required. It is particularly applicable to immunological studies and the culture of embryonic stem cells, insect cells, and smooth muscle cells, as identified by Thermo Fisher.
Published research using FBS
Used in 38 of 68 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- iSsus3744: A Genome-Scale Model-Guided Strategy for Rational Media Design for Cultivated Pork
- Engineered marble-like bovine fat tissue for cultured meat
- Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
- Decellularized spinach: An edible scaffold for laboratory-grown meat
- Scale-up of an intensified bioprocess for the expansion of bovine adipose-derived stem cells (bASCs) in stirred tank bioreactors
Studies from Cellag Bio, a database of published cultivated meat research.
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