Customising Chassis Cells for Structured Meat Products
For cultivated meat R&D teams, producing structured whole-cuts like steaks or fillets requires more than just growing cells. The key lies in chassis cells - muscle, fat, and connective tissue...
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Gibco | SKU:
Gibco™ Trypsin-EDTA (0.25%), with phenol red
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£21.00
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In stock. Ships globally direct from Gibco to research and production facilities.
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Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Gibco to ensure successful implementation.
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Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Gibco™ Trypsin-EDTA is a proteolytic enzyme solution used for the dissociation and detachment of adherent mammalian cells from culture vessels. This formulation contains 0.25% trypsin and 0.5 mM EDTA, providing effective cell release while preserving cell viability.
This specific formulation (Cat. No. 25200056) includes phenol red as a pH indicator, allowing for easy monitoring of solution pH. Produced in cGMP-compliant facilities, this solution is sterile-filtered (0.2 μm) and manufactured to meet rigorous quality standards, ensuring consistent performance.
Trypsin cleaves adhesion proteins, while EDTA chelates divalent cations that facilitate cell-cell and cell-substrate adhesion. These components work synergistically to enhance dissociation efficiency, making this solution suitable for a variety of adherent cell lines, including primary cells and immortalized cell lines.
Supplied in 100 mL bottles, this solution should be stored at –20°C. After thawing, store at 2–8°C and use within one month. Avoid repeated freeze-thaw cycles to maintain enzymatic activity. Inactivate trypsin with serum-containing medium or trypsin inhibitor. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Regulatory compliancecGMP
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Chemically definedYes
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Concentration
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Media category
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Phenol redYes
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Media FormLiquid
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Common name
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Reagent category
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Serum Type
Cellag Bio is the structured intelligence layer for cultivated meat R&D — scattered published research distilled into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before committing budget to a medium, reagent, or cell line, check the evidence behind it: real experimental outcomes and failure modes, not marketing claims.
Search by media, reagent, cell line or species (e.g. "Beefy-9" or "B8") to find the records that use it.
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