Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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HiMedia Dulbecco's Modified Eagle Medium (DMEM), High glucose
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
DMEM, high glucose · Proliferation
Protocol Specified
MyoWorks Chicken Primary Muscle Cells
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with DMEM ShowHide Components that studies in Cellag Bio used alongside DMEM, and where to find them on Cellbase.Includes FBS, Horse serum, FGF2, DMEM/F12.
Bovine · Muscle cells (9)Avian · Muscle cells (5)Bovine · Connective tissue cells (3)Bovine · Fat cells (3)
FBSUsed in 22 of 28 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
Horse serumUsed in 11 of 28 Cellag Bio studies
Gibco™ Horse Serum, New Zealand Origin
FGF2Used in 10 of 28 Cellag Bio studies
Qkine Recombinant Eel FGF-2 (145 Aa) Protein
DMEM/F12Used in 6 of 28 Cellag Bio studies
Gibco™ DMEM/F-12
Also used in these studies, not currently on Cellbase: Matrigel, Gelatin. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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HiMedia Dulbecco’s Modified Eagle Medium (DMEM), High Glucose is a widely used basal medium designed to support the growth of a variety of mammalian cells. This formulation is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of amino acids and vitamins, with additional glycine, serine, and ferric nitrate, providing a nutrient-rich environment suitable for high-density cell culture.
This specific formulation (Cat. No. AL007A) contains 4.5 g/L glucose (high glucose), L-glutamine, sodium pyruvate, and phenol red. The sodium bicarbonate buffer system requires a 5–10% CO₂ environment to maintain physiological pH. The high glucose concentration supports cells with high metabolic demands, including primary cultures of mouse and chicken cells, as well as various normal and transformed cell lines.
Quality control testing ensures consistent performance: appearance is a red-colored clear solution, pH ranges from 7.00 to 7.60, osmolality is 320–360 mOsm/kg, and endotoxin content is ≤1 EU/mL. The medium is sterile-filtered and tested for bacterial and fungal growth with no growth observed after 14 days of incubation per USP specifications.
Supplied as a 1X ready-to-use liquid, this medium should be stored at 2–8°C away from bright light. Shelf life is 12 months from the date of manufacture. Use before the expiry date indicated on the product label. Users are advised to review literature for recommendations regarding medium supplementation specific to different cell lines. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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Animal origin freeYes
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Buffer system
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Chemically definedYes
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Concentration
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Do not freezeYes
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Endotoxin level (EU/mL)
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Glucose (mg/L)
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Media category
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Osmolality (mOsm/kg)
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pH Max
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pH Min
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Phenol redYes
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Serum content
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Xeno‑freeYes
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Media FormLiquid
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L-glutamineYes
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Sodium PyruvateYes
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Appearance
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Light SensitivityYes
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For Research Use OnlyYes
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Temperature Max (°C)
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Temperature Min (°C)
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Mycoplasma Status
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Basal Medium
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Sterility Test Status
Published research using DMEM
Used in 28 of 68 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- Non-ammoniagenic proliferation and differentiation media for cultivated adipose tissue
- Engineered marble-like bovine fat tissue for cultured meat
- Simplification of culture conditions and feeder-free expansion of bovine embryonic stem cells
- Engineered whole cut meat-like tissue by the assembly of cell fibers using tendon-gel integrated bioprinting
- Spent media analysis suggests cultivated meat media will require species and cell type optimization
Studies from Cellag Bio, a database of published cultivated meat research.
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