Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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Opo Bio | SKU:
Opo-Moo-M18
Opo Bio Bovine Angus Satellite Cells
Regular price
£681.00
Unit price
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Unavailable
(Plus Shipping - Calculated in Cart)
Supplier protocol
What the supplier's protocol for these cells calls for, and where to find it on Cellbase.
Basal medium
DMEM/F-12, HEPES
Protocol Specified
Gibco™ DMEM/F-12, HEPES
Serum
FBS
Protocol Specified
Gibco™ Fetal Bovine Serum
Growth factors
bFGF
Protocol Specified
Invitrogen Human FGF-basic (FGF-2/bFGF) Recombinant Protein
Antibiotic
Penicillin-Streptomycin
Protocol Specified
Gibco™ Penicillin-Streptomycin
Antimycotic
Amphotericin B
Protocol Specified
Gibco™ Amphotericin B
Basal medium
DMEM, high glucose, GlutaMAX, pyruvate
Protocol Specified
Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate
Direct Substitute
KCell DMEM
Use with GlutaMAX and sodium pyruvate as an alternative to the specified medium.
Serum
Horse serum
Protocol Specified
Gibco™ Horse Serum, New Zealand Origin
Supplements
GlutaMAX (with KCell DMEM)
Direct Substitute
Gibco™ GlutaMAX™ Supplement
Add to KCell DMEM, with sodium pyruvate, as an alternative to the specified medium.
Sodium pyruvate (with KCell DMEM)
Direct Substitute
Gibco™ Sodium Pyruvate (100 mM)
Add to KCell DMEM, with GlutaMAX, as an alternative to the specified medium.
Antibiotic
Penicillin-Streptomycin
Protocol Specified
Gibco™ Penicillin-Streptomycin
Dissociation
TrypLE Express
Protocol Specified
Gibco™ TrypLE™ Express Enzyme (1X) - No Phenol Red
Cryoprotectant
DMSO
Protocol Specified
Invitrogen™ DMSO, Anhydrous
Suppliers usually provide their full protocol, with volumes and usage instructions, after purchase. Ask us if you need details before ordering →
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Direct Substitute: assessed by Cellbase's technical team as equivalent for the same job.
Check specifications with each supplier before ordering.
What published studies used with these cells ShowHide Components that studies in Cellag Bio used with cells like these, and where to find them on Cellbase.Includes DMEM/F12, DMEM, FGF2, NRG1.
Based on 14 Cellag Bio studies
Basal medium
DMEM/F12Used in 8 of 14 Cellag Bio studies
Gibco™ DMEM/F-12
DMEMUsed in 3 of 14 Cellag Bio studies
Gibco™ DMEM, high glucose
Serum or serum alternative
Also used in these studies, not currently on Cellbase: Albumin, Rapeseed protein isolate. Ask us to source these →
Growth factors
FGF2Used in 8 of 14 Cellag Bio studies
Bovine FGF-basic (FGF-2/bFGF), Animal-Free Recombinant Protein, PeproTech®
NRG1Used in 4 of 14 Cellag Bio studies
Qkine Recombinant Bovine/Porcine NRG-1 Protein
TGF-β3Used in 4 of 14 Cellag Bio studies
XCeLL Factor™ Recombinant TGF-β3 Human
Other options (1)
CultureGro™ Human TGF-beta 3 Animal Free Recombinant Protein
Supplements
InsulinUsed in 4 of 14 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Sodium selenite. Ask us to source this →
Based on 11 Cellag Bio studies
Basal medium
DMEMUsed in 4 of 11 Cellag Bio studies
Gibco™ DMEM, high glucose
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Opo Bio to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Opo Bio with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Opo Bio to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
- Card (secure processing, immediate fulfilment)
- Bank transfer (proforma invoice issued at checkout)
- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Opo Bio bovine cell lines provide reliable primary cell sources for cultivated beef research and development. Each line is isolated from defined cattle breeds, including Aberdeen Angus, offering consistency and traceability for comparative studies. The cells are derived from adult male skeletal muscle tissue and supplied frozen at low passage. They maintain stable growth and differentiation potential across multiple passages, providing a strong foundation for muscle tissue engineering.
All lines are adherent and validated for myogenic marker expression through antibody staining and FACS analysis. Typical doubling times average thirty-three hours, with post-thaw viability above ninety percent. Each vial contains approximately 1.2 million viable cells and has been tested negative for bacteria, fungi, and mycoplasma by a certified laboratory. Sourcing from high-health status New Zealand livestock ensures a pathogen-free profile, including BSE-free certification, to support global regulatory submissions.
Opo Bio cells are supplied with a full documentation pack that includes donor information, morphology images, recommended culture media, and differentiation protocols. Access to technical support and verified procedures is included with each purchase to facilitate seamless integration into existing R&D workflows.
These products are for research and development use only. They are not intended for human or diagnostic applications.
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Regulatory complianceBSE-free
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Scale compatibilityPilot scale
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Storage temperature
-
Validation status
-
Cold Chain Class
-
Temperature Max (°C)
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Temperature Min (°C)
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Cell count per vial
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Cryopreservation viability (%)
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Anchorage TypeAdherent
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Origin country
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Primary vs immortalized
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Species
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Tissue type
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Mycoplasma Status
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Serum Type
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GMO Status
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Breed
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Donor Age
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Donor Sex
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Doubling Time (hours)
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Passage at Banking
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Marker Expression ConfirmedYes
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Thawing Protocol AvailableYes
Published research
Studies on the same species and cell type in Cellag Bio.
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
- Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells
- A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation
Studies from Cellag Bio, a database of published cultivated meat research.
Checkout Options
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Bank Transfer or Purchase Order
Select bank transfer at checkout to receive a proforma invoice. Purchase orders accepted. Your order processes upon payment receipt.
Your supplier ships your order and invoices you directly for the goods.
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