Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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Components that studies in Cellag Bio used alongside FGF2, and where to find them on Cellbase.
Capricorn Scientific Fetal Bovine Serum (FBS)
Defined Bioscience DMEM/F12 Basal Medium
KCell DMEM
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Matrigel, Ascorbic acid 2-phosphate. Ask us to source these →
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Qkine's research grade bovine/porcine FGF-2 is a species-matched fibroblast growth factor essential for maintaining stem cell proliferation, self-renewal, and myoblast expansion in cultivated meat cell culture. Species-matched growth factors deliver superior receptor binding efficiency compared to heterologous proteins, enabling lower supplementation concentrations, improved cellular responses, and enhanced cost-effectiveness in serum-free media formulations for bovine/porcine cell lines.
This research-grade recombinant protein is produced in E. coli expression systems and purified to >95% purity by SDS-PAGE quantitative densitometry. The protein is animal-origin-free, carrier protein-free, and tag-free to ensure high purity and consistent performance across batches. Comprehensive quality control includes mass spectrometry verification, bioactivity testing, endotoxin analysis (<0.05 EU/μg), and batch-specific documentation including certificates of analysis.
This protein is supplied lyophilized and should be reconstituted in 10 mM HCl or phosphate buffer at concentrations above 50 µg/mL, with optional addition of carrier protein for enhanced stability. Single-use aliquots are recommended to maintain protein integrity, with storage at -20°C for short-term use or -80°C for long-term storage. Suitable for research and development applications including cell line development, media optimization, process feasibility studies, and proof-of-concept experiments. For research use only; not intended for human or animal consumption.
Used in 26 of 68 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
Studies from Cellag Bio, a database of published cultivated meat research.
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