Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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Quest | SKU:
Quest Bovine Primary Cell Lines - Aberdeen Angus Cross Myosatellite
Regular price
£1,000.00
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Supplier protocol
What the supplier's protocol for these cells calls for, and where to find it on Cellbase.
Basal medium
DMEM, high glucose
Protocol Specified
Corning® DMEM High Glucose
Serum
FBS
Protocol Specified
Sigma-Aldrich Fetal Bovine Serum
Growth factors
FGF2 (bovine, animal-free)
Protocol Specified
Bovine FGF-basic (FGF-2/bFGF), Animal-Free Recombinant Protein, PeproTech®
Protocol Specified
Protevo Bio bFGF2 – Bovine Fibroblast Growth Factor 2
Protocol Specified
Qkine Recombinant Bovine/Porcine FGF-2 (145 Aa) Protein
Buffer
PBS (without Ca and Mg)
Protocol Specified
Corning® Phosphate-Buffered Saline (PBS)
Dissociation
TrypLE Select
Protocol Specified
Gibco™ TrypLE™ Select Enzyme (1X), no phenol red
Cryoprotectant
DMSO
Protocol Specified
Sigma-Aldrich Dimethyl Sulfoxide (DMSO)
Attachment and coating
Laminin-511 E8 substrate
Protocol Specified
Merck Millipore ECMatrix™-511 E8 Laminin Substrate
Suppliers usually provide their full protocol, with volumes and usage instructions, after purchase. Ask us if you need details before ordering →
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with these cells ShowHide Components that studies in Cellag Bio used with cells like these, and where to find them on Cellbase.Includes DMEM/F12, DMEM, FGF2, NRG1.
Based on 14 Cellag Bio studies
Basal medium
DMEM/F12Used in 8 of 14 Cellag Bio studies
Gibco™ DMEM/F-12
DMEMUsed in 3 of 14 Cellag Bio studies
Gibco™ DMEM, high glucose
Serum or serum alternative
Also used in these studies, not currently on Cellbase: Albumin, Rapeseed protein isolate. Ask us to source these →
Growth factors
FGF2Used in 8 of 14 Cellag Bio studies
Bovine FGF-basic (FGF-2/bFGF), Animal-Free Recombinant Protein, PeproTech®
NRG1Used in 4 of 14 Cellag Bio studies
Qkine Recombinant Bovine/Porcine NRG-1 Protein
TGF-β3Used in 4 of 14 Cellag Bio studies
XCeLL Factor™ Recombinant TGF-β3 Human
Other options (1)
CultureGro™ Human TGF-beta 3 Animal Free Recombinant Protein
Supplements
InsulinUsed in 4 of 14 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Sodium selenite. Ask us to source this →
Based on 11 Cellag Bio studies
Basal medium
DMEMUsed in 4 of 11 Cellag Bio studies
Gibco™ DMEM, high glucose
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Quest to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Quest with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Quest to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
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- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Quest Bovine Aberdeen Angus Cross Myosatellite cells provide a reliable, traceable primary cell source for cultivated beef R&D. These cells enable researchers to establish stable muscle tissue engineering foundations using defined cattle breeds, facilitating the transition from benchtop experiments to pilot-scale production.
Isolated from muscle tissue of Aberdeen Angus Cross cattle and supplied at passage one to ensure high differentiation potential. Each batch is provided with a comprehensive documentation pack including donor-specific traceability, morphology imagery, and established differentiation protocols to ensure experimental reproducibility.
Validated for myogenic marker expression via antibody staining with typical doubling times under 40 hours. Post-thaw viability exceeds 70% with stable growth maintained across multiple passages. All vials are certified negative for bacteria, fungi, and mycoplasma by independent laboratory testing.
Adherent cells supplied as a frozen suspension in one-million-cell vials. Requires standard myogenic culture media and adherent surfaces for expansion; no complex adaptation periods are necessary for implementation into existing workflows.
Available under research or commercial licenses for applications ranging from academic R&D to pilot-scale cultivated meat product development.
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Regulatory complianceISO 9001, GMP
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Scale compatibilityLab scale
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Storage temperature
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Cold Chain Class
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Temperature Max (°C)
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Temperature Min (°C)
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Authentication method
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Cell count per vial
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Cell line name
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Cryopreservation viability (%)
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Differentiation capacity
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Anchorage TypeAdherent
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Origin country
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Passage Range
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Primary vs immortalized
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Species
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Tissue type
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Viability (%)
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Mycoplasma Status
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Basal Medium
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Coating RequiredYes
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Recommended Coating
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CO2 Percent
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Biosafety Level
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GMO Status
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License TypeR&D
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COA File
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Breed
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Doubling Time (hours)
Published research
Studies on the same species and cell type in Cellag Bio.
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
- Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells
- A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation
Studies from Cellag Bio, a database of published cultivated meat research.
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Select bank transfer at checkout to receive a proforma invoice. Purchase orders accepted. Your order processes upon payment receipt.
Your supplier ships your order and invoices you directly for the goods.
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