Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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Gibco | SKU:
Gibco™ TrypLE™ Express Enzyme (1X) - No Phenol Red
Regular price
£30.00
Unit price
/
Unavailable
(Plus Shipping - Calculated in Cart)
Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
TrypLE Express · Passaging
Protocol Specified
Opo Bio Bovine Angus Satellite Cells
Opo Bio Bovine Angus Fibroblasts
Trypsin · Passaging
Direct Substitute
MyoWorks Chicken Primary Muscle Cells
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Direct Substitute: assessed by Cellbase's technical team as equivalent for the same job.
Check specifications with each supplier before ordering.
Availability
Availability
Ships globally direct from Gibco to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Gibco with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Gibco to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
- Card (secure processing, immediate fulfilment)
- Bank transfer (proforma invoice issued at checkout)
- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
TrypLE™ Express is a recombinant cell-dissociation enzyme designed as a superior alternative to porcine trypsin. Derived from microbial fermentation, this animal-origin free (AOF) reagent effectively dissociates a wide range of adherent mammalian cells, including CHO, HEK 293, A549, and primary keratinocytes. Its gentle action preserves cell surface proteins and ensures high viability, making it ideal for sensitive downstream applications where maintaining cellular integrity is paramount.
This enzyme is manufactured in a dedicated animal-origin-free, cGMP-compliant facility and is processed under strict ISO 13485 standards. Each lot is validated for sterility, pH, and osmolality, ensuring exceptional purity and performance consistency. Unlike traditional trypsin, TrypLE™ Express is highly stable at room temperature, eliminating the need for frequent freeze-thaw cycles and simplifying laboratory workflows.
The formulation is phenol red-free and ready-to-use (1X), requiring no reconstitution or dilution. Its ease of use and ability to be inactivated by simple dilution—rather than requiring specialized trypsin inhibitors—makes it a dependable choice for both research and large-scale bioprocessing. It is particularly well-suited for serum-free culture systems where animal-derived components must be avoided.
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Regulatory compliancecGMP, ISO 13485
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Scale compatibilityLab scale
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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Animal origin freeYes
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Chemically definedYes
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Do not freezeYes
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Phenol redYes
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Xeno‑freeYes
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Media FormLiquid
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Temperature Max (°C)
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Temperature Min (°C)
Cellag Bio has no published studies on this product yet.
Cellag Bio is a database of published cultivated meat research, organized into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before you commit budget to a medium, reagent, or cell line, check the evidence behind it.
Search by medium, reagent, cell line, or species (for example "Beefy-9" or "B8") to find the studies that use it.
Search the Cellag Bio database →
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Select bank transfer at checkout to receive a proforma invoice. Purchase orders accepted. Your order processes upon payment receipt.
Your supplier ships your order and invoices you directly for the goods.
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