LCA Data for Cultivated Meat Equipment Selection
For cultivated meat bioprocess engineers, I compare equipment impacts per 1 kg of usable finished product - not by rated power. I first compare bioreactor systems for process fit, food...
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Gibco | SKU:
31550023
Gibco™ Ham's F-10 Nutrient Mix
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
Ham's F-10 · Proliferation
Protocol Specified
WildBio Zebra Myoblast Cell Line – Muscle
WildBio Springbok Myoblast Cell Line – Muscle
WildBio Blue Wildebeest Myoblast Cell Line – Muscle
Used as a 50:50 mix with DMEM, per the supplier protocol.
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with Ham's F-10 ShowHide Components that studies in Cellag Bio used alongside Ham's F-10, and where to find them on Cellbase.Includes FBS, DMEM, FGF2, Horse serum.
Bovine · Muscle cells (6)Avian · Muscle cells (3)Porcine · Muscle cells (3)Fish and seafood · Muscle cells (1)
FBSUsed in 11 of 11 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
DMEMUsed in 9 of 11 Cellag Bio studies
Gibco™ DMEM, high glucose
FGF2Used in 9 of 11 Cellag Bio studies
Qkine Recombinant Eel FGF-2 (145 Aa) Protein
Horse serumUsed in 4 of 11 Cellag Bio studies
Gibco™ Horse Serum, New Zealand Origin
Also used in these studies, not currently on Cellbase: Collagen I, Matrigel. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Gibco to research and production facilities.
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Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Gibco to ensure successful implementation.
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Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
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Your local duties & taxes are payable where applicable.
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Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Gibco™ Ham's F-10 Nutrient Mix was originally developed for the serum-free growth of Chinese Hamster Ovary (CHO) cells and has since become a widely used basal medium for a variety of mammalian cell culture applications. In addition to supporting CHO cell growth, it has been successfully used with other cell types, including COS-7 cells, primary rat astrocytes, and rat prostate epithelial cells.
Compared to many basal media, Ham's F-10 contains a broader range of nutrients and trace components, including zinc, hypoxanthine, and thymidine. These additional components help support cellular growth and maintenance in specialized culture systems and have contributed to the development of improved formulations such as Ham's F-12 Nutrient Mixture.
This specific formulation (Cat. No. 31550023) contains L-glutamine and phenol red and does not contain HEPES. The medium contains no proteins, lipids, hormones, growth factors, or serum and is typically supplemented according to the requirements of the cultured cell type.
Manufactured under stringent quality standards, Gibco™ Ham's F-10 Nutrient Mix is sterile-filtered and designed to provide consistent performance for research and cell culture workflows.
For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Regulatory complianceISO 13485
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Shelf life (months)
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Storage temperature
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Validation status
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Cold Chain Class
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Buffer system
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Concentration
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Glucose (mg/L)
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Media category
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Osmolarity Min Mosm Kg
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Osmolarity Max Mosm Kg
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pH Max
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pH Min
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Phenol redYes
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Sterile filter pore size (µm)
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Media FormLiquid
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L-glutamineYes
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Sodium PyruvateYes
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Light SensitivityYes
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For Research Use OnlyYes
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Temperature Max (°C)
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Temperature Min (°C)
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Basal Medium
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CO2 Percent
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Certificate of Analysis AvailableYes
Published research using Ham's F-10
Used in 11 of 112 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- Engineered whole cut meat-like tissue by the assembly of cell fibers using tendon-gel integrated bioprinting
- Spent media analysis suggests cultivated meat media will require species and cell type optimization
- Effect of Serum and Oxygen on the In Vitro Culture of Hanwoo Korean Native Cattle-Derived Skeletal Myogenic Cells Used in Cellular Agriculture
- Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat
- The Effects of Laxogenin and 5-Alpha-hydroxy-laxogenin on Myotube Formation and Maturation During Cultured Meat Production
Studies from Cellag Bio, a database of published cultivated meat research.
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