Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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Gibco | SKU:
Gibco™ Trypsin-EDTA (0.25%), with phenol red
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£21.00
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Unavailable
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
Trypsin-EDTA (0.25%) · Passaging
Protocol Specified
WildBio Blesbok Fibroblast Cell Line – Skin
WildBio Blue Wildebeest Fibroblast Cell Line – Skin
WildBio Springbok Fibroblast Cell Line – Skin
Other cell lines (22)
WildBio Giraffe Fibroblast Cell Line – Skin
WildBio Impala Fibroblast Cell Line – Skin
WildBio Ostrich Fibroblast Cell Line – Skin
WildBio Sable Antelope Fibroblast Cell Line – Skin
WildBio Springbok MSC Cell Line – Bone Marrow
WildBio Buffalo MSC Cell Line – Bone Marrow
WildBio Giraffe MSC Cell Line – Bone Marrow
WildBio Ostrich MSC Cell Line – Bone Marrow
WildBio Sable Antelope MSC Cell Line – Bone Marrow
WildBio Impala Myoblast Cell Line – Muscle
WildBio Zebra MSC Cell Line – Bone Marrow
WildBio Zebra Myoblast Cell Line – Muscle
WildBio Ostrich Myoblast Cell Line – Muscle
WildBio Giraffe Myoblast Cell Line – Muscle
WildBio Giraffe Pre-Adipocyte Cell Line – Fat
WildBio Springbok Myoblast Cell Line – Muscle
WildBio Springbok Pre-Adipocyte Cell Line – Fat
WildBio Blue Wildebeest Myoblast Cell Line – Muscle
WildBio Buffalo Pre-Adipocyte Cell Line – Fat
WildBio Duiker Pre-Adipocyte Cell Line – Fat
WildBio Impala Pre-Adipocyte Cell Line – Fat
Livestock Labs Porcine Fibroblasts
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
Availability
Availability
Ships globally direct from Gibco to research and production facilities.
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Shipping
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Orders fulfilled directly by Gibco with temperature-controlled shipping where required.
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Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Gibco to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
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Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Gibco™ Trypsin-EDTA is a proteolytic enzyme solution used for the dissociation and detachment of adherent mammalian cells from culture vessels. This formulation contains 0.25% trypsin and 0.5 mM EDTA, providing effective cell release while preserving cell viability.
This specific formulation (Cat. No. 25200056) includes phenol red as a pH indicator, allowing for easy monitoring of solution pH. Produced in cGMP-compliant facilities, this solution is sterile-filtered (0.2 μm) and manufactured to meet rigorous quality standards, ensuring consistent performance.
Trypsin cleaves adhesion proteins, while EDTA chelates divalent cations that facilitate cell-cell and cell-substrate adhesion. These components work synergistically to enhance dissociation efficiency, making this solution suitable for a variety of adherent cell lines, including primary cells and immortalized cell lines.
Supplied in 100 mL bottles, this solution should be stored at –20°C. After thawing, store at 2–8°C and use within one month. Avoid repeated freeze-thaw cycles to maintain enzymatic activity. Inactivate trypsin with serum-containing medium or trypsin inhibitor. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Regulatory compliancecGMP
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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Chemically definedYes
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Concentration
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Phenol redYes
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Media FormLiquid
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Common name
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Reagent category
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For Research Use OnlyYes
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Temperature Max (°C)
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Temperature Min (°C)
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Serum Type
Cellag Bio has no published studies on this product yet.
Cellag Bio is a database of published cultivated meat research, organized into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before you commit budget to a medium, reagent, or cell line, check the evidence behind it.
Search by medium, reagent, cell line, or species (for example "Beefy-9" or "B8") to find the studies that use it.
Search the Cellag Bio database →
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