DMEM vs F12 for Cultivated Meat Cell Lines
For cultivated meat R&D teams, I’d screen DMEM, Ham’s F12 and a 1:1 DMEM/F12 mix before choosing a basal medium. None is complete on its own: your cell line, supplements...
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KCell | SKU:
KCell Leibovitz’s L-15
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£9.00
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Cell line pairings
Cell lines this product is paired with on Cellbase. The label shows the basis for each pairing.
L-15 · Proliferation
Direct Substitute
MOR Cell.Bio Marbled Goby MUSC
Use as an alternative to the specified medium. Not validated by MORCell Bio.
How we choose these
Direct Substitute: assessed by Cellbase's technical team as equivalent for the same job.
Check specifications with each supplier before ordering.
What published studies used with L-15 ShowHide Components that studies in Cellag Bio used alongside L-15, and where to find them on Cellbase.Includes FBS, FGF2, DMEM, Horse serum.
Fish and seafood · Connective tissue cells (4)Fish and seafood · Muscle cells (4)Fish and seafood · {"muscle"=>"Muscle cells", "fat"=>"Fat cells", "connective"=>"Connective tissue cells", "stem"=>"Stem cells", "msc"=>"Mesenchymal stem cells", "pluripotent"=>"Pluripotent stem cells"} (3)Fish and seafood · Pluripotent stem cells (2)
FBSUsed in 13 of 13 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
FGF2Used in 4 of 13 Cellag Bio studies
Bovine FGF-basic (FGF-2/bFGF), Animal-Free Recombinant Protein, PeproTech®
DMEMUsed in 3 of 13 Cellag Bio studies
KCell DMEM
Horse serumUsed in 3 of 13 Cellag Bio studies
Gibco™ Horse Serum, New Zealand Origin
IGF-1Used in 2 of 13 Cellag Bio studies
Qkine Recombinant Bovine/Porcine IGF-1 Protein
Other options (1)
CultureGro™ Human IGF-1 Animal Free Recombinant Protein
Also used in these studies, not currently on Cellbase: Gelatin. Ask us to source this →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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KCell Leibovitz’s L-15 Medium is formulated for the cultivation of cells in non-CO₂ environments. It replaces sodium bicarbonate with phosphate buffering and uses free-base amino acids to maintain physiological pH, allowing cultures to thrive in sealed or open-air conditions. L-15 supports a wide range of mammalian cell types, including fibroblasts, epithelial cells, and tissue explants, and is particularly suited to field or transport applications where CO₂ control is limited.
Produced under ISO and cGMP standards, KCell Leibovitz’s L-15 offers reliable composition and batch reproducibility. It is supplied in liquid or powder form, with optional inclusion of L-glutamine, phenol red, and sodium pyruvate to accommodate different experimental setups. Each batch is validated for sterility, pH stability, and osmolarity before distribution.
The medium’s self-buffering chemistry provides stable pH and nutrient conditions across extended culture periods, making it ideal for cultivated-meat, tissue-engineering, and general mammalian cell-culture workflows performed outside conventional incubator environments.
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Shelf life (months)
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Storage temperature
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Cold Chain Class
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Buffer additives allowedYes
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Buffer system
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Do not freezeYes
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Endotoxin level (EU/mL)
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Glucose (mg/L)
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Media category
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Non‑CO₂ Buffer Option
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Optimized for
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Osmolality (mOsm/kg)
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pH Max
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pH Min
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Phenol redYesYes--YesYes--
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Serum content
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L-glutamineYesYes--YesYes--
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Sodium PyruvateYesYesYesYesYesYesYesYes
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Temperature Max (°C)
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Temperature Min (°C)
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Basal Medium
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Cell Line SpecificityAvian, Bovine, Seafood, Porcine
Published research using L-15
Used in 13 of 157 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- Novel Eel Skin Fibroblast Cell Line: Bridging Adherent and Suspension Growth for Aquatic Applications Including Virus Susceptibility
- Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla
- Myogenic Response to Increasing Concentrations of Ammonia Differs between Mammalian, Avian, and Fish Species: Cell Differentiation and Genetic Study
- Decellularized banana leaves: eco-friendly scaffolds for cell-based seafood
- Assessment of plant- and microbial-derived protein hydrolysates as sustainable for fetal bovine serum in seafood cell culture media
Studies from Cellag Bio, a database of published cultivated meat research.
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Insights & News
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Scaffold Pore Size Analysis: Equipment Selection Checklist
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Non-Animal Scaffolds Under Heat and pH Stress
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LCA Data for Cultivated Meat Equipment Selection
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Serum-Free Media Design for Myoblast Cell Lines
For myoblast culture teams, I would select a serum-free medium by two outcomes: viable cell expansion and retained myotube-forming capacity. Start with your cell line’s history and measurable acceptance limits...
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Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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UCOE vs cHS4 for Stable Gene Expression
For cultivated meat R&D teams, I would choose UCOE or cHS4 by measured expression retention - not initial expression strength. Compare matched constructs with an unprotected control across independent clones....
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Spent Media Regeneration: Batch vs Continuous Routes
If you run cultivated meat process development, the choice is simple in principle: batch gives tighter lot isolation; continuous cuts hold time and smooths media return - but only if...
