LCA Data for Cultivated Meat Equipment Selection
For cultivated meat bioprocess engineers, I compare equipment impacts per 1 kg of usable finished product - not by rated power. I first compare bioreactor systems for process fit, food...
1
of
6
Loading...
Quest | SKU:
Quest Bovine Primary Cell Lines - Limousine Myosatellite
Regular price
£1,000.00
Unit price
/
Unavailable
(Plus Shipping - Calculated in Cart)
Supplier protocol
What the supplier's protocol for these cells calls for, and where to find it on Cellbase.
Basal medium
DMEM, high glucose
Protocol Specified
Corning® DMEM High Glucose
Serum
FBS
Protocol Specified
Sigma-Aldrich Fetal Bovine Serum
Growth factors
FGF2 (bovine, animal-free)
Protocol Specified
Bovine FGF-basic (FGF-2/bFGF), Animal-Free Recombinant Protein, PeproTech®
Protocol Specified
Protevo Bio bFGF2 – Bovine Fibroblast Growth Factor 2
Protocol Specified
Qkine Recombinant Bovine/Porcine FGF-2 (145 Aa) Protein
Buffer
PBS (without Ca and Mg)
Protocol Specified
Corning® Phosphate-Buffered Saline (PBS)
Dissociation
TrypLE Select
Protocol Specified
Gibco™ TrypLE™ Select Enzyme (1X), no phenol red
Cryoprotectant
DMSO
Protocol Specified
Sigma-Aldrich Dimethyl Sulfoxide (DMSO)
Attachment and coating
Laminin-511 E8 substrate
Protocol Specified
Merck Millipore ECMatrix™-511 E8 Laminin Substrate
Suppliers usually provide their full protocol, with volumes and usage instructions, after purchase. Ask us if you need details before ordering →
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with these cells ShowHide Components that studies in Cellag Bio used with cells like these, and where to find them on Cellbase.Includes DMEM/F12, DMEM, Ham's F-10, FGF2.
Based on 28 Cellag Bio studies
Basal medium
DMEM/F12Used in 12 of 28 Cellag Bio studies
Gibco™ DMEM/F-12
DMEMUsed in 11 of 28 Cellag Bio studies
Gibco™ DMEM, high glucose
Ham's F-10Used in 3 of 28 Cellag Bio studies
Gibco™ Ham's F-10 Nutrient Mix
Serum or serum alternative
Also used in these studies, not currently on Cellbase: Albumin, Fetuin, Rapeseed protein isolate. Ask us to source these →
Growth factors
FGF2Used in 14 of 28 Cellag Bio studies
Bovine FGF-basic (FGF-2/bFGF), Animal-Free Recombinant Protein, PeproTech®
NRG1Used in 4 of 28 Cellag Bio studies
Qkine Recombinant Bovine/Porcine NRG-1 Protein
Also used in these studies, not currently on Cellbase: HGF. Ask us to source this →
Based on 22 Cellag Bio studies
Basal medium
DMEMUsed in 13 of 22 Cellag Bio studies
Gibco™ DMEM, high glucose
DMEM/F12Used in 2 of 22 Cellag Bio studies
Gibco™ DMEM/F-12
Supplements
Also used in these studies, not currently on Cellbase: Oleic acid. Ask us to source this →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Quest to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Quest with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Quest to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
- Card (secure processing, immediate fulfilment)
- Bank transfer (proforma invoice issued at checkout)
- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Quest Bovine Limousine Myosatellite cell lines provide a reliable primary cell source for cultivated beef research and development. These cells are isolated from Limousine bovine muscle tissue and supplied frozen at passage one. They maintain stable growth and differentiation potential across multiple passages, providing a strong foundation for muscle tissue engineering.
The myosatellite cell lines are adherent and validated for myogenic marker expression through antibody staining. Typical doubling times range from less than thirty to forty hours depending on the cell type, with post-thaw viability above seventy percent. Each vial contains one million viable cells and has been tested negative for bacteria, fungi, and mycoplasma by a certified laboratory. The cells are supplied with a full documentation pack that includes donor information, morphology images, recommended culture media, and differentiation protocols.
Quest bovine cell lines are available under two license options. The research license allows use in academic and R&D environments. The commercial license grants rights for pilot-scale and product development activities. Both are one-time fees that include access to protocol updates and technical support.
These products are for research and development use only. They are not intended for human or diagnostic applications.
-
Regulatory complianceISO 9001, GMP
-
Scale compatibilityLab scale
-
Storage temperature
-
Cold Chain Class
-
Temperature Max (°C)
-
Temperature Min (°C)
-
Authentication method
-
Cell count per vial
-
Cell line name
-
Cryopreservation viability (%)
-
Differentiation capacity
-
Anchorage TypeAdherent
-
Origin country
-
Passage Range
-
Primary vs immortalized
-
Species
-
Tissue type
-
Viability (%)
-
Mycoplasma Status
-
Basal Medium
-
Coating RequiredYes
-
Recommended Coating
-
CO2 Percent
-
Biosafety Level
-
GMO Status
-
License TypeR&D
-
COA File
-
Breed
-
Doubling Time (hours)
Published research
Studies on the same species and cell type in Cellag Bio.
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
- Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells
- A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation
Studies from Cellag Bio, a database of published cultivated meat research.
Checkout Options
Card
Secure payment processing. Card details are not stored.
Bank Transfer or Purchase Order
Select bank transfer at checkout to receive a proforma invoice. Purchase orders accepted. Your order processes upon payment receipt.
Your supplier ships your order and invoices you directly for the goods.
Shop
Insights & News
View all-
-
Serum-Free Media Design for Myoblast Cell Lines
For myoblast culture teams, I would select a serum-free medium by two outcomes: viable cell expansion and retained myotube-forming capacity. Start with your cell line’s history and measurable acceptance limits...
-
Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
-
UCOE vs cHS4 for Stable Gene Expression
For cultivated meat R&D teams, I would choose UCOE or cHS4 by measured expression retention - not initial expression strength. Compare matched constructs with an unprotected control across independent clones....
-
Spent Media Regeneration: Batch vs Continuous Routes
If you run cultivated meat process development, the choice is simple in principle: batch gives tighter lot isolation; continuous cuts hold time and smooths media return - but only if...
-
Gene Knockout Design for Cultivated Meat Cell Lines
If a knockout does not improve a defined process trait, it is just an edit, not a usable cell line. For bioprocess engineers, cell culture scientists, and cultivated meat R&D...
-
TEA vs LCA in Waste System Uncertainty
If you use TEA and LCA to judge the same cultivated meat waste route, you should expect different answers. That is not a modelling failure. It happens because TEA tests...
-
Trace Element Tuning for Muscle Cell Lines
If you tune trace elements by label dose alone, you can miss what muscle cells actually see. In muscle-derived cultivated meat media, the gap between nominal and delivered concentration can...
