Non-Animal Scaffolds Under Heat and pH Stress
For cultivated meat R&D teams, I assess scaffold stability by retained function - not appearance. Test your formulation through its actual heating, cooling and pH sequence. For adherent culture, check...
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R&D Systems Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein - With Carrier
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What published studies used with FGF2
Components that studies in Cellag Bio used alongside FGF2, and where to find them on Cellbase.
Bovine · Muscle cells (24)Porcine · Muscle cells (7)Bovine · Pluripotent stem cells (6)Avian · Muscle cells (4)
FBSUsed in 38 of 54 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
DMEMUsed in 30 of 54 Cellag Bio studies
Gibco™ DMEM, high glucose
DMEM/F12Used in 23 of 54 Cellag Bio studies
Gibco™ DMEM/F-12
Horse serumUsed in 12 of 54 Cellag Bio studies
Gibco™ Horse Serum, New Zealand Origin
Also used in these studies, not currently on Cellbase: Matrigel, Gelatin. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Recombinant Human FGF-basic (FGF-2/bFGF) is a highly bioactive signaling protein essential for the culture and maintenance of diverse mammalian cell types. This 146 amino acid variant is a potent mitogen that stimulates the proliferation of cells of mesodermal and neuroectodermal origin. It is a critical component in media formulations for pluripotent stem cells (PSCs), neural stem cells, and endothelial cells, playing a vital role in maintaining pluripotency and directing lineage-specific differentiation.
This growth factor is expressed in E. coli and purified to exceptional standards, typically exceeding 95% purity as determined by SDS-PAGE and visualized by silver stain. Each lot is rigorously validated for biological activity using a cell proliferation assay with Quiescent NR6R-3T3 fibroblasts, ensuring an ED50 typically within 0.1–0.6 ng/mL. The lyophilized formulation is carrier-free, providing researchers with a clean, high-performance reagent for sensitive applications in regenerative medicine and cancer research.
The 146 aa FGF-basic is ideal for both foundational research and advanced bioprocessing where precise control over cell signaling is required. Known for its robust performance across multiple cell types, it is a dependable choice for wound healing studies, angiogenesis assays, and stem cell expansion. For maximum stability, the protein should be reconstituted according to validated protocols and stored in working aliquots at -20°C to -70°C to avoid repeated freeze-thaw cycles.
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Scale compatibilityLab scale
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Contains BSAYes
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For Research Use OnlyYes
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Species
Published research using FGF2
Used in 54 of 157 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
- Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells
- Decellularized spinach: An edible scaffold for laboratory-grown meat
Studies from Cellag Bio, a database of published cultivated meat research.
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