Non-Animal Scaffolds Under Heat and pH Stress
For cultivated meat R&D teams, I assess scaffold stability by retained function - not appearance. Test your formulation through its actual heating, cooling and pH sequence. For adherent culture, check...
1
of
5
Loading...
Cellcraft | SKU:
CELL-POR-FIB-HDR
Cellcraft Spontaneously Immortalized Porcine Preadipocyte Cells
Regular price
£20,000.00
Unit price
/
Unavailable
(Plus Shipping - Calculated in Cart)
Supplier protocol
What the supplier's protocol for these cells calls for, and where to find it on Cellbase.
Proliferation
Basal medium
DMEM/F12
Protocol Specified
Defined Bioscience DMEM/F12 Basal Medium
Protocol Specified
Gibco™ DMEM/F-12
Serum
FBS
Protocol Specified
Gibco™ Fetal Bovine Serum
Growth factors
FGF2 (heat stable)
Protocol Specified
Defined Bioscience FGF2-G3 Growth Factor (Bovine)
Protocol Specified
Qkine Recombinant Bovine/Porcine FGF2-G3 (154 Aa) Protein
Suppliers usually provide their full protocol, with volumes and usage instructions, after purchase. Ask us if you need details before ordering →
How we choose these
Protocol Specified: named in the cell line supplier's protocol, either this exact product or another brand where the supplier says brand doesn't matter.
Check specifications with each supplier before ordering.
What published studies used with these cells ShowHide Components that studies in Cellag Bio used with cells like these, and where to find them on Cellbase.Includes FGF2, Laminin, DMEM, DMEM/F12.
Based on 1 Cellag Bio study · Limited evidence
Growth factors
FGF2Used in 1 of 1 Cellag Bio study
Qkine Recombinant Bovine/Porcine FGF-2 (145 Aa) Protein
Attachment and coating
LamininUsed in 1 of 1 Cellag Bio study
Merck Millipore ECMatrix™-511 E8 Laminin Substrate
Based on 3 Cellag Bio studies
Basal medium
DMEMUsed in 1 of 3 Cellag Bio studies
Gibco™ DMEM, high glucose
DMEM/F12Used in 1 of 3 Cellag Bio studies
Gibco™ DMEM/F-12
Supplements
Also used in these studies, not currently on Cellbase: Intralipid, Biotin, Calcium pantothenate, Dexamethasone. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
Availability
Availability
Ships globally direct from Cellcraft to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Cellcraft with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Licences
Licences
Live cell shipments across borders might require an import permit for your country. Your supplier will confirm the exact requirements for your destination before shipping.
Support
Support
Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Cellcraft to ensure successful implementation.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
- Card (secure processing, immediate fulfilment)
- Bank transfer (proforma invoice issued at checkout)
- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
A spontaneously immortalized porcine preadipocyte cell line isolated from the subcutaneous fat of Sus scrofa via enzymatic tissue digestion. These cells represent an immature precursor lineage committed to adipocyte development, naturally residing in adipose tissue where they maintain the capacity to proliferate and differentiate into mature, lipid-accumulating fat cells upon receiving specific hormonal signals.
The line was established through spontaneous immortalization. This process resulted in a stable proliferative capacity and continuous growth performance without the need for genetic modifications.
Cells are supplied in a 1.8 mL cryovial at a density of 1 × 10⁶ cells/mL (Lot rWCB01) and must be stored in the vapor phase of liquid nitrogen. The cell line is confirmed negative for Mycoplasma and common pathogens, with a guaranteed post-thaw viability of >70%. Phenotypic characterization via flow cytometry confirms the line is >90% positive for CD90 and contains a ~25% subpopulation of FAP (fibroblast activated protein) positive cells, while remaining negative (<5%) for CD34, CD45, and CD31.
Documentation is provided, including brightfield phase microscopy imagery and fluorescent labeling data of neutral lipids following 10 days in adipogenic medium to confirm differentiation potential.
The listed purchase price includes a 1 year research license (£20,000/year), & 2 cell vials. Additional vials will cost £200 + shipping.
-
Storage temperature
-
Cold Chain Class
-
Temperature Max (°C)
-
Temperature Min (°C)
-
Certification
-
Cell count per vial
-
Cell line name
-
Differentiation capacity
-
Anchorage TypeAdherent
-
Passage Range
-
Primary vs immortalized
-
Species
-
Tissue type
-
Viability (%)
-
Immortalization Method
-
Mycoplasma Status
-
Basal Medium
-
Serum Type
-
Biosafety Level
-
GMO Status
-
Doubling Time (hours)
Published research
Studies on the same species and cell type in Cellag Bio.
- A simplified and defined serum-free medium for cultivating fat across species
- Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
- Modulation of nutritional composition and aroma volatiles in cultivated pork fat by culture media supplementation
Studies from Cellag Bio, a database of published cultivated meat research.
Checkout Options
Card
Secure payment processing. Card details are not stored.
Bank Transfer or Purchase Order
Select bank transfer at checkout to receive a proforma invoice. Purchase orders accepted. Your order processes upon payment receipt.
Your supplier ships your order and invoices you directly for the goods.
Shop
Insights & News
View all-
-
LCA Data for Cultivated Meat Equipment Selection
For cultivated meat bioprocess engineers, I compare equipment impacts per 1 kg of usable finished product - not by rated power. I first compare bioreactor systems for process fit, food...
-
Serum-Free Media Design for Myoblast Cell Lines
For myoblast culture teams, I would select a serum-free medium by two outcomes: viable cell expansion and retained myotube-forming capacity. Start with your cell line’s history and measurable acceptance limits...
-
Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
-
UCOE vs cHS4 for Stable Gene Expression
For cultivated meat R&D teams, I would choose UCOE or cHS4 by measured expression retention - not initial expression strength. Compare matched constructs with an unprotected control across independent clones....
-
Spent Media Regeneration: Batch vs Continuous Routes
If you run cultivated meat process development, the choice is simple in principle: batch gives tighter lot isolation; continuous cuts hold time and smooths media return - but only if...
-
Gene Knockout Design for Cultivated Meat Cell Lines
If a knockout does not improve a defined process trait, it is just an edit, not a usable cell line. For bioprocess engineers, cell culture scientists, and cultivated meat R&D...
-
TEA vs LCA in Waste System Uncertainty
If you use TEA and LCA to judge the same cultivated meat waste route, you should expect different answers. That is not a modelling failure. It happens because TEA tests...
