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Cellcraft  |  SKU: CELL-POR-FIB-HDR

Cellcraft Spontaneously Immortalised Porcine Preadipocyte Cells

£20,000.00

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A spontaneously immortalized porcine preadipocyte cell line isolated from the subcutaneous fat of Sus scrofa via enzymatic tissue digestion. These cells represent an immature precursor lineage committed to adipocyte development, naturally residing in adipose tissue where they maintain the capacity to proliferate and differentiate into mature, lipid-accumulating fat cells upon receiving specific hormonal signals.

The line was established through prolonged in vitro culture, achieving immortalization spontaneously after surpassing the Hayflick limit and successfully navigating a subsequent crisis phase. This process resulted in a stable proliferative capacity and continuous growth performance without the need for exogenous immortalizing agents or genetic modifications. Growth kinetics captured over 40 passages (~140 doublings) demonstrate a robust and consistent average doubling time of 21 hours.

Cells are supplied in a 1.8 mL cryovial at a density of 1 × 10⁶ cells/mL (Lot rWCB01) and must be stored in the vapor phase of liquid nitrogen. The cell line is confirmed negative for Mycoplasma and common pathogens, with a guaranteed post-thaw viability of >70%. Phenotypic characterization via flow cytometry confirms the line is >90% positive for CD90 and contains a ~25% subpopulation of FAP (fibroblast activated protein) positive cells, while remaining negative (<5%) for CD34, CD45, and CD31.

Comprehensive documentation is provided, including brightfield phase microscopy imagery and fluorescent labeling data of neutral lipids following 10 days in adipogenic medium to confirm differentiation potential. The recommended culture environment utilizes DMEM/F12 supplemented with 10% FBS and 10ng/mL FGF2 to maintain optimal adherent growth.

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