Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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XCeLL | SKU:
XCeLL Factor™ Recombinant FGF2 Bovine Porcine
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What published studies used with FGF2
Components that studies in Cellag Bio used alongside FGF2, and where to find them on Cellbase.
Bovine · Muscle cells (13)Bovine · Pluripotent stem cells (5)Porcine · Muscle cells (4)Avian · Muscle cells (3)
FBSUsed in 15 of 26 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
DMEM/F12Used in 12 of 26 Cellag Bio studies
Defined Bioscience DMEM/F12 Basal Medium
DMEMUsed in 10 of 26 Cellag Bio studies
KCell DMEM
InsulinUsed in 5 of 26 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Matrigel, Ascorbic acid 2-phosphate. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from XCeLL to ensure successful implementation.
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Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
XCeLL Factor™ Recombinant FGF2 Bovine/Porcine is a potent, heparin-binding recombinant protein engineered to regulate cell proliferation, survival, migration, and differentiation in mammalian cell systems. By activating FGFR signaling pathways, it promotes angiogenesis and supports the growth of fibroblasts, stem cells, and primary cell lines, making it an essential component for serum-free formulations and cell culture research. Expressed in an E. coli host system, this protein corresponds to UniProt ID P03969.
The protein consists of an amino acid length of 185 AA (including His tag) with a molecular weight of approximately ~20.4 kDa. Quality specifications ensure >95% purity as determined by SDS-PAGE, an endotoxin level below 0.1 EU/µg, and 0.2 µm sterile filtration. Functional testing in an NIH-3T3 fibroblast proliferation assay demonstrates dose-dependent activity with an ED50 of approximately ≤1.08 ng/mL.
Offered in both lyophilized and liquid formats under catalog number XCF-FGF-002, the product is shipped on dry ice or gel packs. Reconstitution is recommended in sterile water or PBS to a concentration of 0.1–0.5 mg/mL. For optimal stability, store the lyophilized protein at -20°C to -80°C. Reconstituted solutions remain stable for up to 1 week at 2–8°C or long-term as frozen aliquots at -20°C. This product is intended strictly for research use only.
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Scale compatibilityProduction scale
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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After mix stability (max days)
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Aliquoting recommendedYes
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Animal origin freeYes
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Chemically definedYes
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Endotoxin level (EU/mL)
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Optimized for
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Post‑thaw stability (max days)
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Sterile filter pore size (µm)
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Xeno‑freeYes
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Media FormLyophilized
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Chemical name
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Common name
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Hazard classificationNone
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Molecular weight (g/mol)
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Purity (%)
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Reagent category
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Solubility
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For Research Use OnlyYes
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Cell Line SpecificityBovine, Porcine
Published research using FGF2
Used in 26 of 68 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
- Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
- Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells
- Decellularized spinach: An edible scaffold for laboratory-grown meat
Studies from Cellag Bio, a database of published cultivated meat research.
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