Secondary Fermentation of Spent Media Byproducts
For cultivated meat R&D teams, my starting rule is simple: accept, condition or reject spent media before inoculation, based on batch composition and safety limits - not the original medium...
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XCeLL | SKU:
XCeLL Factor™ Recombinant FGF2 Gallus
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What published studies used with FGF2
Components that studies in Cellag Bio used alongside FGF2, and where to find them on Cellbase.
Bovine · Muscle cells (13)Bovine · Pluripotent stem cells (5)Porcine · Muscle cells (4)Avian · Muscle cells (3)
FBSUsed in 15 of 26 Cellag Bio studies
Capricorn Scientific Fetal Bovine Serum (FBS)
DMEM/F12Used in 12 of 26 Cellag Bio studies
Defined Bioscience DMEM/F12 Basal Medium
DMEMUsed in 10 of 26 Cellag Bio studies
KCell DMEM
InsulinUsed in 5 of 26 Cellag Bio studies
XCeLL Factor™ Recombinant Human Insulin
Also used in these studies, not currently on Cellbase: Matrigel, Ascorbic acid 2-phosphate. Ask us to source these →
How we choose these
These come from published studies in Cellag Bio. They show which components those studies used, not these exact products, and results can differ in your system. Components not yet on Cellbase are listed at the end of each section. Check specifications and compatibility with each supplier.
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XCeLL Factor™ Recombinant FGF2 Gallus from Cell Culture Collective is a high-purity, heparin-binding recombinant protein engineered to support cell proliferation, migration, stem cell expansion, and tissue regeneration in avian cell culture systems. Produced via an E. coli expression system, this chemically defined growth factor plays an essential role in maintaining primary cell lines and satellite cells, making it an excellent component for cultivated meat research and serum-free formulation strategies.
The protein features an amino acid length of 189 AA (with His tag) and an estimated molecular weight of ~20.7 kDa. It undergoes rigorous quality control to deliver >95% purity as evaluated by SDS-PAGE, an endotoxin level of <0.1 EU/µg, and a 0.2 µm sterile-filtered rating. In functional validation assays utilizing NIH-3T3 fibroblast proliferation, it demonstrates dose-dependent activity with an ED50 of ≤1 ng/mL.
Available in flexible lyophilized or liquid formats under catalog number XCF-FGF-003, the protein is shipped on dry ice or gel packs. For long-term stability, lyophilized protein should be stored between -20°C and -80°C. Reconstitution in sterile water or PBS to a concentration of 0.1–0.5 mg/mL allows stable storage at 2–8°C for up to one week, or long-term storage in frozen aliquots at -20°C. This product is intended for research use only.
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Scale compatibilityProduction scale
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Cold Chain Class
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After mix stability (max days)
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Aliquoting recommendedYes
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Animal origin freeYes
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Chemically definedYes
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Endotoxin level (EU/mL)
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Optimized for
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Post‑thaw stability (max days)
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Sterile filter pore size (µm)
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Xeno‑freeYes
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Media FormLyophilized
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Chemical name
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Common name
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Hazard classificationNone
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Molecular weight (g/mol)
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Purity (%)
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Reagent category
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Solubility
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For Research Use OnlyYes
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Temperature Max (°C)
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Temperature Min (°C)
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Cell Line SpecificityAvian
Published research using FGF2
Used in 26 of 68 Cellag Bio studies.
These studies used this component, not necessarily this exact product.
- Spent media analysis suggests cultivated meat media will require species and cell type optimization
- The Effects of Laxogenin and 5-Alpha-hydroxy-laxogenin on Myotube Formation and Maturation During Cultured Meat Production
- A Comparative Study on the Taste Characteristics of Satellite Cell Cultured Meat Derived from Chicken and Cattle Muscles
- A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates
- A simple and robust serum-free media for the proliferation of muscle cells
Studies from Cellag Bio, a database of published cultivated meat research.
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