Worlds First Cultivated Meat B2B Marketplace: Read Announcement
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Gibco™ Trypsin-EDTA is a proteolytic enzyme solution used for the dissociation and detachment of adherent mammalian cells from culture vessels. This formulation contains 0.25% trypsin and 0.5 mM EDTA, providing effective cell release while preserving cell viability.
This specific formulation (Cat. No. 25200056) includes phenol red as a pH indicator, allowing for easy monitoring of solution pH. Produced in cGMP-compliant facilities, this solution is sterile-filtered (0.2 μm) and manufactured to meet rigorous quality standards, ensuring consistent performance.
Trypsin cleaves adhesion proteins, while EDTA chelates divalent cations that facilitate cell-cell and cell-substrate adhesion. These components work synergistically to enhance dissociation efficiency, making this solution suitable for a variety of adherent cell lines, including primary cells and immortalized cell lines.
Supplied in 100 mL bottles, this solution should be stored at –20°C. After thawing, store at 2–8°C and use within one month. Avoid repeated freeze-thaw cycles to maintain enzymatic activity. Inactivate trypsin with serum-containing medium or trypsin inhibitor. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
Cellag Bio is the structured intelligence layer for cultivated meat R&D — scattered published research distilled into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before committing budget to a medium, reagent, or cell line, check the evidence behind it: real experimental outcomes and failure modes, not marketing claims.
Search by media, reagent, cell line or species (e.g. "Beefy-9" or "B8") to find the records that use it.
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Size
Regulatory compliance
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Shelf life (months)
Storage temperature
Validation status
Variant structure
Product Sterilisation Method
After mix stability (max days)
After mix stability (min days)
Aliquoting recommended
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Buffer additives allowed
Buffer system
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Complete media storage temperature
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Contains animal components
Contains BSA
Do not freeze
Do not refilter
Endotoxin level (EU/mL)
Food grade status
Glucose (mg/L)
Media category
Mix immediately after thaw
Non‑CO₂ Buffer Option
Optimized for
Osmolality (mOsm/kg)
Osmolarity (mOsm/kg)
pH Max
pH Min
Phenol red
Post‑thaw stability (max days)
Post‑thaw stability (min days)
Refreezing allowed
Serum content
Sterile filter pore size (µm)
Xeno‑free
Media Form
L-glutamine
Sodium Pyruvate
HEPES
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CAS number
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Chemical structure image
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DO control
Edible components
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Usage Guidance Available
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Size Range (mm)
Lattice Parameters
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Certified for food use
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Tested cell lines
Therapeutic grade available
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Sterilization compatible
Parameter
Diameter mm
Process Connection
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Output Sensor
Temperature Sensor Type
Wetted Parts
Measurement Principle
Optical Path Length mm
Wavelength nm
Electrolyte
Regulatory Approval
Measuring Range
Configurable Values
Conductivity Range mS/cm
Accuracy at 25°C
Precision at 25°C
Certification
Operating Temp Min °C
Operating Temp Max °C
Operating Voltage VDC
Pressure Range bar g
IP Rating
Autoclavable
Steam Sterilizable SIP
CIP Compatible
Single Use Option
Authentication method
Cell count per vial
Cell line name
Cryopreservation viability (%)
Differentiation capacity
Anchorage Type
Origin country
Passage Range
Primary vs immortalized
Species
Tissue type
Viability (%)
Immortalization Method
Mycoplasma Status
Culture Mode Detail
Basal Medium
Serum Type
Coating Required
Recommended Coating
CO2 Percent
Biosafety Level
GMO Status
MTA Required
License Type
COA File
Breed
Species Detail
Donor Age
Donor Sex
Food-Safe Status
Doubling Time
Karyotype Data Available
Pathogen Screening
Donor Health Status
Tissue Collection Method
Authentication Status
Last Authentication Date
Mycoplasma Test Method
Sterility Test Status
Viral Screening Status
Passage at Banking
Max Passages Before Plateau
Differentiation Validation Method
Marker Expression Confirmed
Recommended Seeding Density
Thawing Protocol Available
Passaging Protocol Available
Availability Status
Population Doubling Limit
Growth Plateau Indicator
PDL Before Plateau
Storage Format
Humidity Required
Spinner Flask Drive
Impeller Design
Side Port Count
Cap Type
Bottle Thread
Coating Method
Coating Stability
Mesh Count
Prefilter Included
Color Coding
Harvest Arm Present
