KCell DMEM/F12

£8.00

(Plus Shipping - Calculated in Checkout)

Composition

With Glucose – With L-Glutamine & Phenol Red With Glucose – Without L-Glutamine & Phenol Red Glucose-Free – With L-Glutamine & Phenol Red Glucose-Free – Without L-Glutamine & Phenol Red

Size

500 mL 1000 mL

Product variants

Request Volume Quote Here

Availability

In stock. Ships globally direct from KCell to research and production facilities.

Shipping

Add to cart & view shipping options / prices in checkout for your region.

Orders fulfilled directly by KCell with temperature-controlled shipping where required.

Your local duties & taxes are payable where applicable.

Support

Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from KCell to ensure successful implementation.

Payment

Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com

Payment methods supported

Card (secure processing, immediate fulfilment)
Bank transfer (proforma invoice issued at checkout)
Purchase order (submit PO number with order or via email)

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VAT handled per vendor jurisdiction.

Your local duties & taxes are payable where applicable.

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Legal

Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.

DMEM/F12 combines Dulbecco’s Modified Eagle Medium with Ham’s F-12 nutrient mix at a 1:1 ratio, providing a balanced formulation widely used for mammalian, endothelial, and fibroblast cell culture. KCell DMEM/F12 offers a broad nutrient spectrum with optimised amino acid, vitamin, and trace-element content to support high viability and reproducible growth across diverse cell types.

Each lot is produced under cGMP and ISO-13485 standards to ensure consistency and regulatory reliability. Variants include differences in glucose concentration, L-glutamine, phenol red, and buffering systems, allowing users to match the formulation to specific process conditions. The medium can be supplied as a ready-to-use liquid or as a dry powder for bulk preparation.

KCell DMEM/F12 is commonly used in serum-free and reduced-serum systems for cultivated-meat research, particularly during scale-up or differentiation phases. The balanced formulation provides the nutrient stability required for long-term culture and complex co-culture systems.

Shelf life (months)

12
12
12
12
12
12
12
12
Storage temperature

2-8°C
Buffer additives allowed

Yes
Buffer system

Custom
Do not freeze

Yes
Endotoxin level (EU/mL)

<1
Glucose (mg/L)

3,200
3,200
3,200
3,200
0
0
0
0
Media category

Basal
Non‑CO₂ Buffer Option

HEPES
Optimized for

Widely used basal medium; examples include MDCK, glial, fibroblast, human endothelial, and rat fibroblast cells
Osmolality (mOsm/kg)

320-360
pH Max

8.2
pH Min

7.5
Phenol red

Yes
Yes
-
-
Yes
Yes
-
-
Serum content

Serum-free
L-glutamine

Yes
Yes
-
-
Yes
Yes
-
-
Sodium Pyruvate

Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes

Checkout Options

Card

Secure payment processing. Card details are not stored.

Bank Transfer or Purchase Order

Select bank transfer at checkout to receive a proforma invoice. Purchase orders accepted. Your order processes upon payment receipt.

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