Customising Chassis Cells for Structured Meat Products
For cultivated meat R&D teams, producing structured whole-cuts like steaks or fillets requires more than just growing cells. The key lies in chassis cells - muscle, fat, and connective tissue...
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Gibco | SKU:
11330032
Gibco™ DMEM/F-12, HEPES
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£20.00
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Availability
In stock. Ships globally direct from Gibco to research and production facilities.
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Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Gibco with temperature-controlled shipping where required.
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Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Gibco to ensure successful implementation.
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Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
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Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Gibco™ DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium designed to support the growth of a broad range of mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. This 1:1 mixture combines the high concentrations of glucose, amino acids, and vitamins from DMEM with the wide variety of components found in Ham’s F-12.
This specific formulation is modified with L-glutamine, HEPES, and phenol red. The addition of HEPES provides effective buffering to ensure pH stability, while the sodium bicarbonate system requires a 5–10% CO₂ environment to maintain physiological conditions. Produced in cGMP-compliant facilities, Gibco™ DMEM/F-12 contains no proteins, lipids, or growth factors.
Each batch is manufactured to meet rigorous quality standards for sterility and performance, making this medium an ideal foundation for serum-free media systems or custom supplementations (commonly 10% fetal bovine serum).
This medium is stable for up to 12 months when stored at 2–8°C and protected from light. It is shipped at ambient temperature and is suitable for both research use and further manufacturing applications. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Regulatory compliancecGMP, ISO 13485
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Shelf life (months)
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Storage temperature
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Validation status
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Product Sterilisation Method
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Animal origin freeYes
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Concentration
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Glucose (mg/L)
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Phenol redYes
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L-glutamineYes
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HEPESYes
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Appearance
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CO2 Percent
Cellag Bio is the structured intelligence layer for cultivated meat R&D — scattered published research distilled into decision-ready records: what worked, what failed, the likely cause, and what to do instead. Before committing budget to a medium, reagent, or cell line, check the evidence behind it: real experimental outcomes and failure modes, not marketing claims.
Search by media, reagent, cell line or species (e.g. "Beefy-9" or "B8") to find the records that use it.
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