Energy Efficiency in Bioreactor Scale-Up Processes
Scaling up bioreactors for cultivated meat production - from small (1–5 L) to large (1,000+ L) systems - brings energy challenges. Larger volumes require more power for mixing, oxygen transfer,...
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Defined Bioscience | SKU:
Defined Bioscience HiDef® B8 Stem Cell Growth Medium
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£112.00
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Availability
In stock. Ships globally direct from Defined Bioscience to research and production facilities.
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Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
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Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Support
Support
Defined Bioscience provides direct post-sales support including troubleshooting, process optimisation, and protocol guidance. Batch-specific documents available on request.
Defined Bioscience’s HiDef® B8 Stem Cell Growth Medium is a complete, chemically defined formulation designed for feeder-free maintenance and expansion of human pluripotent stem cells. It provides a reliable foundation for cultivated-meat research by supporting long-term proliferation of mammalian stem and progenitor cells under serum-free conditions. The medium maintains cells in an undifferentiated state while reducing labour demands through a flexible feeding schedule that enables weekend-free culture.
HiDef B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and a thermostable variant of FGF2. This combination eliminates the need for additional supplementation while maintaining genomic stability and morphology for over fifty passages. The formulation has been validated across multiple human iPSC lines and is compatible with a wide range of matrices including Matrigel®, Geltrex™, and vitronectin substrates.
Available as a complete medium kit or as a supplement-only format for use with DMEM/F12 basal media, HiDef B8 offers full flexibility for process development. Once prepared aseptically, the complete medium can be stored at 2–8 °C for two weeks and supports consistent growth without karyotypic drift.
For research and development use only. Not for human or diagnostic applications.
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Regulatory compliance------
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Scale compatibilityProduction scale
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Shelf life (months)
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Sterility method
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Storage temperature
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Validation status
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After mix stability (max days)
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Aliquoting recommendedYes
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Animal origin freeYes
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Buffer additives allowedYes
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Chemically definedYes
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Concentration
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Do not freezeYes
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Endotoxin level (EU/mL)
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Media categorySupplementSupplementSupplementSupplementSupplementSupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, SupplementBasal, Supplement
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Mix immediately after thawYes
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Non‑CO₂ Buffer Option
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Optimized for
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Post‑thaw stability (max days)
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Serum content
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Sterile filter pore size (µm)
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Xeno‑freeYes
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Media FormLiquid
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Light SensitivityYes
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