Energy Efficiency in Bioreactor Scale-Up Processes
Scaling up bioreactors for cultivated meat production - from small (1–5 L) to large (1,000+ L) systems - brings energy challenges. Larger volumes require more power for mixing, oxygen transfer,...
1
of
2
Loading...
Defined Bioscience | SKU:
Defined Bioscience HiDef® ITS Cell Culture Supplement
Regular price
£53.00
Unit price
/
Unavailable
(Plus Shipping - Calculated in Checkout)
Request Volume Quote Here
Availability
Availability
In stock. Ships globally direct from Defined Bioscience to research and production facilities.
Shipping
Shipping
Add to cart & view shipping options / prices in checkout for your region.
Orders fulfilled directly by Defined Bioscience with temperature-controlled shipping where required.
Your local duties & taxes are payable where applicable.
Payment
Payment
Purchase orders also accepted. Select bank transfer at checkout and provide your PO number in order notes or email to sales@cellbase.com
- Card (secure processing, immediate fulfilment)
- Bank transfer (proforma invoice issued at checkout)
- Purchase order (submit PO number with order or via email)
VAT handled per vendor jurisdiction.
Your local duties & taxes are payable where applicable.
Card payments process immediately. Bank transfer and PO orders process upon payment receipt.
Legal
Legal
Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.
Support
Support
Defined Bioscience provides direct post-sales support including troubleshooting, process optimisation, and protocol guidance. Batch-specific documents available on request.
Defined Bioscience’s HiDef® ITS Cell Culture Supplement is a chemically defined, animal-origin-free serum replacement used to maintain and support adherent cells under reduced-serum or serum-free conditions. It combines three critical components normally supplied by serum—insulin, transferrin, and sodium selenite—each optimised to sustain cell metabolism, attachment, and viability across a wide range of primary, immortalised, and stem cell types.
Insulin stimulates the uptake of glucose, amino acids, and ions to promote cell growth and prevent apoptosis. Transferrin serves as a high-affinity iron carrier and antioxidant, reducing toxicity associated with free iron exposure. Sodium selenite contributes antioxidant protection and acts as a cofactor for essential selenoenzymes. Together, these factors maintain physiological balance in nutrient-limited conditions and allow foetal bovine serum (FBS) to be reduced to four per cent or less without compromising performance.
HiDef ITS is supplied as a 100× liquid supplement in Earle’s Balanced Salt Solution (EBSS) without phenol red and prepared using fully animal-free components. It is compatible with a wide range of basal media, including Defined Bioscience’s DMEM/F12, and is validated through fibroblast outgrowth and viability testing. Each 10 mL vial is sufficient to prepare one litre of complete medium.
For research and development use only. Not for human or diagnostic applications.
-
Scale compatibilityProduction scale
-
Shelf life (months)
-
Sterility method
-
Storage temperature
-
Validation status
-
After mix stability (max days)
-
After mix stability (min days)
-
Animal origin freeYes
-
Buffer additives allowedYes
-
Buffer system
-
Chemically definedYes
-
Concentration
-
Do not freezeYes
-
Endotoxin level (EU/mL)
-
Glucose (mg/L)
-
Media category
-
Mix immediately after thawYes
-
Optimized for
-
Post‑thaw stability (max days)
-
Post‑thaw stability (min days)
-
Serum content
-
Sterile filter pore size (µm)
-
Xeno‑freeYes
-
Appearance
-
Purity (%)
Checkout Options
Card
Secure payment processing. Card details are not stored.
Bank Transfer or Purchase Order
Select bank transfer at checkout to receive a proforma invoice. Purchase orders accepted. Your order processes upon payment receipt.
Shop
Insights & News
View all-
-
pH and Temperature Control: Impact on Cell Growth
Maintaining precise pH and temperature is critical for growing mammalian cells, especially in cultivated meat production. Cells need a controlled environment to multiply (proliferation) and develop into muscle fibres (differentiation)....
-
Analytical Methods for Live-Cell Monitoring in Bioreactors
Monitoring live cells in bioreactors is critical for cultivated meat production. Scaling requires precise tools to track cell health and growth in real time. This article reviews key methods, including...
-
How to Implement Process Analytical Technology (PAT)
Process Analytical Technology (PAT) integrates real-time quality monitoring into manufacturing processes, improving consistency and reducing waste. It’s especially useful in cultivated meat production, where precise control of factors like pH,...
-
Top 5 Sensors for Cold Chain Monitoring
Maintaining precise temperature and humidity during transport is critical for cultivated meat. Small fluctuations can spoil shipments and lead to waste. Advanced IoT sensors now provide real-time monitoring, ensuring product...
-
Real-Time Media Analysis with Automated Sampling Systems
Automated sampling systems are transforming how bioprocesses are monitored, especially in cultivated meat production. These systems offer frequent, precise, and real-time data on critical factors like nutrient levels, metabolites, and...
-
Scaling Bioreactors: Shear Stress Modelling Techniques
Scaling bioreactors for cultivated meat production is complex, especially when managing shear stress, a mechanical force that can damage mammalian cells during scale-up. Unlike microbial cells, mammalian cells are fragile...
-
Top 7 Decontamination Tools for Cultivated Meat
Contamination is a major hurdle in cultivated meat production, with batch failure rates hitting 11.2% and climbing to 19.5% in larger-scale operations. This not only wastes resources like growth media...
