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Cellcraft  |  SKU: CELL-POR-FIB-HDR

<tc>Cellcraft</tc> Células preadipocíticas porcinas inmortalizadas espontáneamente

£20,000.00

(Plus Shipping - Calculated in Checkout)

Configuración Recomendada

Basado en datos del proveedor y alternativas compatibles para esta línea celular. Vea Notas del Protocolo para más detalles, luego seleccione los componentes requeridos y agréguelos junto con su pedido.

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In stock. Ships globally direct from Cellcraft to research and production facilities.

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Orders fulfilled directly by Cellcraft with temperature-controlled shipping where required.

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Purchase includes full technical documentation (CoAs, Manuals, or Protocols) provided automatically. Direct application support included from Cellcraft to ensure successful implementation.

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Most products require no additional agreements. Cell lines and certain biological materials require a Material Transfer Agreement (MTA) on first purchase from each supplier. We handle this digitally - both parties sign online before shipment. Subsequent orders from the same supplier proceed immediately.

Una línea celular de preadipocitos porcinos inmortalizada espontáneamente, aislada de la grasa subcutánea de Sus scrofa mediante digestión enzimática del tejido. Estas células representan una línea de precursores inmaduros comprometidos con el desarrollo de adipocitos, que residen naturalmente en el tejido adiposo donde mantienen la capacidad de proliferar y diferenciarse en células grasas maduras que acumulan lípidos al recibir señales hormonales específicas.
 
La línea se estableció a través de la inmortalización espontánea. Este proceso resultó en una capacidad proliferativa estable y un rendimiento de crecimiento continuo sin la necesidad de modificaciones genéticas. La cinética de crecimiento capturada durante más de 40 pasajes (~140 duplicaciones) demuestra un tiempo de duplicación promedio robusto y consistente de 21 horas.
 
Las células se suministran en un criovial de 1.8 mL a una densidad de 1 × 10⁶ células/mL (Lote rWCB01) y deben almacenarse en la fase de vapor de nitrógeno líquido.La línea celular se confirma negativa para Mycoplasma y patógenos comunes, con una viabilidad post-descongelación garantizada de >70%. La caracterización fenotípica mediante citometría de flujo confirma que la línea es >90% positiva para CD90 y contiene una subpoblación de ~25% de células positivas para FAP (proteína activada de fibroblastos), mientras que permanece negativa (<5%) para CD34, CD45 y CD31.
 
Se proporciona documentación, incluyendo imágenes de microscopía de fase de campo claro y datos de etiquetado fluorescente de lípidos neutros después de 10 días en medio adipogénico para confirmar el potencial de diferenciación.

El precio de compra listado incluye una licencia de investigación de 1 año (£20,000/año), & 2 viales de células. Viales adicionales costarán £200 + envío

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