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Cellcraft  |  SKU: CELL-POR-FIB-HDR

<tc>Cellcraft</tc> Spontant immortaliserade preadipocyter från gris

£20,000.00

(Plus Shipping - Calculated in Checkout)

Rekommenderad installation

Baserat på leverantörsdata och kompatibla alternativ för denna cellinje. Se Protokollanteckningar för detaljer, välj sedan de nödvändiga komponenterna och lägg till dem tillsammans med din beställning.

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En spontant immortaliserad porcin preadipocytcellinje isolerad från det subkutana fettet hos Sus scrofa via enzymatisk vävnadsnedbrytning. Dessa celler representerar en omogen prekursorlinje som är engagerad i adipocytutveckling, naturligt belägen i fettvävnad där de bibehåller förmågan att proliferera och differentiera till mogna, lipidackumulerande fettceller vid mottagande av specifika hormonella signaler.
 
Linjen etablerades genom spontan immortalisation. Denna process resulterade i en stabil proliferativ kapacitet och kontinuerlig tillväxtprestanda utan behov av genetiska modifieringar. Tillväxtkinetik fångad över 40 passager (~140 fördubblingar) visar en robust och konsekvent genomsnittlig fördubblingstid på 21 timmar.
 
Celler levereras i en 1,8 mL kryorör vid en densitet av 1 × 10⁶ celler/mL (Lot rWCB01) och måste förvaras i ångfasen av flytande kväve.Cellinjen är bekräftad negativ för Mycoplasma och vanliga patogener, med en garanterad post-tiningsviabilitet på >70%. Fenotypisk karakterisering via flödescytometri bekräftar att linjen är >90% positiv för CD90 och innehåller en ~25% subpopulation av FAP (fibroblastaktiverat protein) positiva celler, medan den förblir negativ (<5%) för CD34, CD45 och CD31.
 
Dokumentation tillhandahålls, inklusive ljusfältfasmikroskopibilder och fluorescerande märkdata av neutrala lipider efter 10 dagar i adipogent medium för att bekräfta differentieringspotential.

Det angivna inköpspriset inkluderar en 1-årig forskningslicens (£20,000/år), & 2 cellflaskor. Ytterligare flaskor kostar £200 + frakt

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